A Study on the Toxic Effect of Cadmium on Rat Bones: Electron Probe Micro-Analysis

“我们研究的目的是实验性地证明Cadmium对骨头的直接影响。”在我们的实际研究中，年轻老鼠骨头的机械强度已经明显退化，有人建议，机械强度的退化应该导致骨骼微观结构中的异常变化。为了证明这一点， EPMA技术使用了EPMA技术来分析骨骼元素中的变化，并测试骨骼中的镉定位。年轻老鼠的幽默，在4周内管理为10 ppm的镉，从身体中脱落，固定在-20 ° C的乙醇中，然后驱动并切割到EPMA中的清晰表面。这些样品用金涂层在碳板上，由大市大学工程学院EPM-810（日本Schimazu公司）分析，由EPM分析仪检测到，并由charasteristic X射线波长识别，包括Ca： K<alph>， K<bet>， P： K<alph>， K， K< bet>， Na： K<alph>， K： K<alph>， Mg： K<alph>， S： K<alph>， Cl：K<alph>， Zn： K<alpha>， O：K<alph>， F： K<alph>和Fe： K<alph>。在控制组和镉组中，钙、磷、钠、钾、镁和氯在每个斑点上都可以找到（直径为10<micr>米）。锌、铁和F并没有在任何地方被检测到，但它可以证明这些元素存在于骨头的某些地方。在Cd组的骨骼中，在这些元素的分布中找不到任何变化。在这种方法中很难找到。我们在外延区域检测到Cd定位的小签名，并且没有找到精确站点的喷气机。

The purpose of our study is to prove experimentally the direct effect of cadmium on bones. In our perious study, the mechanical strength of the bones of young rats was clearly decreased and it was suggested that the decrease in the mechanical strength was due to the abnormal change in the microstructure of the bone. In orger to prove this point, EPMA technique was used to analyze the changes in the elements of bones and to examine the cadmium localization in bones.Humeri of young rats, administered with 10ppm of cadmium for 4 weeks, were dissected out from the bodies, fixed in the ethanol at -20゜C, then dried and cut to obtain the clear surface for the EPMA. these samples was gold coated on a carbon plate and analysed by the WDX type-electron probe microanalyzer, EPM-810 (Schimazu Corporation, Japan) in the Faculty of Engineering,Osaka City University.Elements, detected by EPM Analyzer and identified by the charasteristic X ray wavelength, are Ca: K<alph>, K<bet>, P: K<alph>, K< bet>, Na: K<alph>, K: K<alph>, Mg: K<alph>, S: K<alph>, Cl:K<alph>, Zn: K<alpha>, O:K<alph>, F: K<alph> and Fe: K<alph>. Both in the control and Cd groups, Ca, P, Na, K, Mg and Cl are found abundantly on every spot (10<micr>m in diameter).S. Zn, Fe and F are not detected everywhere, but it can be proved that these elements exist in some places in bones. In the bones of Cd group, it is not found any change in the distribution of these elements. Cd is hard to detect in this method. We have detected the small sign of Cd locarization in the epiphysial zone, and not jet found out the precise site of the cadmium localization.