Simultaneous measurements of cerebral blood flow and energy metabolism by 19F and 31P nuclear magnetic resonance spectroscopy.

为了建立在体磁共振波谱同时测量脑血流量和能量代谢的方法，对在体核磁共振波谱仪(JEOL-JNM-GX-270，6.34特斯拉)进行了改进，使之能够自动获得血清19F或1H核磁共振波谱。沙土鼠颈总动脉注射D20，沙土鼠脑内连续监测~1H核磁共振信号，脑内~1H信号在注射D20后短暂减少，之后逐渐恢复到注射前水平。沙土鼠吸入19F氟利昂约10min后，用系列19F核磁共振波谱记录其在沙土鼠脑内的饱和和去饱和度。19F氟利昂毒性小，对动脉血压影响不大。根据19F氟里昂脱饱和曲线计算脑血流量，D20和19F氟里昂清除法测得的脑血流量与常规1jC-安替比林法测得的脑血流量具有良好的相关性。吸入5%CO2时测得的脑血流量比对照组增加了30%，双侧颈总动脉结扎时测得的脑血流量比对照组减少了90%以上。因此，使用活体~1H或~(19)F核磁共振波谱测量脑血流量成为可能。该系统允许同时测量~(31)P核磁共振波谱。利用该系统对脑血流量与能量代谢的关系进行了研究，并对脑和肌肉的细胞内和细胞外Na+及水分进行了测量。

The study was performed to develop method for measuring cerebral blood flow (CBF) and energy metabolism simultaneously by in vivo hmrlear magnetic resonance (NMR) spectroscopy.In vivo NMR spectrometer (JEOL-JNM-GX-270,6.34 Tesla) was modified,so that seroal 19F or 1H NMR spectra were obtained automatically. Using this system,deuterium oxide (D20) clearance or 19F freon (trifluoromethane) clearance was recorded.D20 was injected as a bolus from common carotid artery of mongolian gerbils,and 1H NMR signals were monitored serially in the gerbil brain.1H signals in the brain were reduced transiently following the D20 injection and thereafter recovered gradually towards the pre-injection level. The CBF was Estimatid from this clearance curve.19F freon was inhaled also in mongolian gerbils for about 10 min,and its saturation and desaturation in the gerbil brain were recorded by serial 19F NMR spectroscopy.19F freon has small toxicity providing little influence on arterial blood pressure,blood gases and EEG.The CBF was estimated from the desaturation curve of 19F freon.The CBF values obtained from D20 clearance or 19F freon clearance correlated well with flow values estimated by conventional 1jC-iodoantipyrine methods.The CBF measured during 5% CO2 inhalation was increased by 30% compared with that mesured during control state.The CBF measured during bilateral common carotid artery occlusion was reduced by more than 90% compared with the control values. Thus,using in vivo 1H or 19F NMR spectroscopy,measurement of CBF has become possible.This system allows to measure 31P NMR spectroscopy simultaneously. Therefore,simultaneous measurements of CBF and energy metabolism are possible by this technique.Utilizing this system,the relationship between CBF and energy metabolism was examined.Likewise,measurements of intra-and extracellular na+ and water in the brain and muscle were carried out.