EXPERIMENTAL RESEARCH FOR FETAL SURGERY

本研究旨在对妊娠20 ~ 25天手术产生膈疝的胎兔进行肺发育不全，并探讨胎儿期手术治疗的可能性。手术方法：2.5%硫贲妥钠诱导全身麻醉，50:50氧化亚氮维持，氧添加1.0 ~ 2.0%氟烷。打开母兔腹部，取出子宫角。其中一个胎儿的左前腿和左胸壁通过子宫切开暴露出来。用细止血钳穿过胸壁刺破左膈肌。术后4 ~ 5天行剖宫产。每个胎儿都被称重并牺牲。取出胎儿的双肺，用10%的中性福尔马林溶液固定。在成功产生膈疝的胎兔中发现肺发育不全，组织学表现不那么严重。为了制造更严重的肺发育不良，这种方法应该在更小的胎兔中进行。然而，在妊娠20天前的胎兔上进行这种手术是非常困难的。另一方面，自1988年以来，由于母山羊在手术后2至3天死于肺部并发症，一直在尝试在胎山羊身上建立这种模型。为了完成山羊的这种慢性准备，解决母山羊管理中的一些问题将是非常重要的。

This research was designed to creat hypoplastic lungs on fetal rabbits of 20 to 25 days' gestation with surgically producted diaphragmatic henia and to examine possibility of surgical therapy in fetal period. Surgical techniques: General anesthesia was induced with 2.5% sodium thiopenthal and maintained with 50:50 nitrous oxide and oxygen supplemented by 1.0 to 2.0% halothane. The abdomen of the mother rabbit was opened and the uterine horn was delivered out of the abdomen. The left foreleg and left chest wall of one of the fetuses were exposed through the uterotomy. Left diaphragm was ruptured by penetration of a fine hemostat through the chest wall. The operated fetuses were delivered by cesarean section 4 to 5 days after operation. Each fetus was weighed and sacrificed. Both lungs of the fetuses were removed and fixed in 10% neutral formalin solution. The hypoplastic lungs, which histological findings were not so severe, were recognized in fetal rabbits with successfully producted diaphragmatic hernia. To create more severe hypoplastic lung, this procedure should be carried out in yonger fetal rabbits. However, it was very difficult to do this procedure on fetal rabbits before 20 days' gestation. On the other hand, creation of this model on fetal goats has been tried since 1988 because mother goats died of pulmonary complication 2 to 3 days after surgery. To complete this kind of chronic preparation in goats, it will be very important to kaiketu some problems in management of mother goats.