Biochemical Study on Terminal Oxidase of Bacterial Respiratory Chain

细胞色素氧化酶是一种广泛存在于多种细菌中的酶，通过比较其在大肠杆菌、葡萄糖酸杆菌、醋酸杆菌和假单胞菌中的结构和功能，对细胞色素氧化酶进行了研究。同时，还比较了该氧化酶与其它末端氧化酶在各菌株中的功能。大肠杆菌：呼吸链含有细胞色素o和d作为末端氧化酶，其合成受培养基中氧浓度的调节。细胞色素氧化酶能够与泛醇反应，并在重构的脂蛋白体中产生膜电位。此外，该氧化酶还能与蛋白脂质体中的Q-8和葡萄糖脱氢酶（GDH）重组葡萄糖氧化酶呼吸链。亚呼吸链：由细胞色素o分支的呼吸链和由细胞色素c组成的非能量产生旁路。仅更换了旁路 ...更多信息 介质的pH值。细胞色素o的结构和功能与E.大肠杆菌，从而能够与泛醇反应并产生能量。然而，氧化酶在免疫学上与E.大肠杆菌细胞色素o。此外，该氧化酶还能与Q-10和GDH重组葡萄糖氧化酶呼吸链。aceti：呼吸链含有细胞色素o和a作为末端氧化酶，其根据培养条件（振荡或静止）而变化。细胞色素o具有与上述酶几乎相同的结构和功能。此外，细胞色素a的结构和功能与细胞色素o相似。4）铜绿假单胞菌的末端氧化酶：呼吸链以细胞色素o为末端氧化酶，并有一个对氰化物不敏感的旁路。细胞色素o与其他细胞色素有很大的不同，它与色素c反应，但不与泛醇反应，但在产生能量的性质上没有不同。少

Cytochrome o oxidase, which functions extensively in a wide variety of bacteria, was investigated by comparing its structure and function among several bacteria such as Escherichia, Gluconobacter, Acetobacter and Pseudomonas. The oxidase was also oxidase was also compared in their function with another terminal oxidase coexistion in the respective strains.1) Terminal oxidase of E. coli: The respiratory chain contains cytochromes o and d as terminal oxidases, the synthesis of which is regulated by oxygen concentration in the medium. The cytochrome o oxidase was able to react with ubiquinol, and to generate a membrane potential in the reconstituted proteoliposomes. Furthermore, the oxidase was able to reconstitute glucose oxidase respiratory chain with Q-8 and glucose dehydrogenase (GDH) in the proteoliposomes.2) Terminal oxidase of G. subboxydans: The respiratory chain branched with cytochrome o and a non-energy-generating bypass consisting of a cytochrome c. The bypass only was changed … More by pH of the medium. The cytochrome o had almost the same structure and function as that of E. coli, and thus was able to react with ubiquinol and to generate an energy. However, the oxidase was somewhat different immunologically from e. coli cytochrome o. Furthermore, the oxidase was also able to reconstitute glucose oxidase respiratory chain with Q-10 and GDH.3) Terminal oxidase of A. aceti: The respiratory chain contained cytochromes o and a as terminal oxidases, which changed depending on culture conditions, shaking or stationary. The cytochrome o had almost the same structure and function as the enzyme described above. Furthermore, the structure and function of the cytochrome a was similar to those of the cytochrome o. At this moment, however, the relationship between the cytochromes o and a is not clear.4) Terminal oxidase of P. aerugiosa: The respiratory chain had cytochrome o as the terminal oxidase, and further a cyanide insensitive bypass. The cytochrome o was extensively different from others, reacting chtochrome c but not with ubiquinol, but not different in the property to generate an energy. Less