Genetic monitoring of domestic animals at the DNA level.

限制性内切酶的线粒体DNA的切割模式进行了分析，在菲律宾人口的本地牛。在由下列酶BamHI、BglII、EcoRV、HindIII、PstI和ScaI产生的限制性图谱中检测到mtDNA多态性，表明存在两种不同类型的mtDNA。这两种mtDNA在亚种水平上有很大差异，菲律宾本地牛被认为是欧洲牛和印度牛的混合体。人cDNA有时不适合家畜，特别是鸡。因此，用鸡基因组DNA的2- 2.5kb EcoRI片段和pUC 12载体进行随机克隆。筛选出28个独立克隆，其中4个克隆用于RFLP检测。在本研究所检测的14个品种中均发现了RFLP位点。

An analysis of patterns of cleavage of mtDNA by restriction endonucleases was performed in the Philippine population of native cattle. MtDNA polymorphisms were detected in the restriction patterns generated by the following enzymes, BamHI, BglII, EcoRV, HindIII,, PstI and ScaI, indicating the existence of two separate types of mtDNA. These two types of mtDNA are very different from each other at the level of subspecies, and the native Philippine cattle are considered to represent an admixture of European and Indian cattle.Restriction fragment length polymorphism (RFLP) was examined in several domestic animals using human MHC class II cDNA as probe. Human cDNA is not sometimes suitable for domestic animals, especially chicken. Therefore, a random cloning was performed with 2-2.5 kb EcoRI fragments of chicken genome DNA and pUC12 vectors. 28 independent clones were selected and 4 clones were used for the detection of RFLPs. The RFLPs were distrivuted in the 14 kinds of breed examined in this study.