Detection of mycoplasma antibodies and an attempt of simple identification by the dot-immunobinding reactions.

研究了斑点免疫结合（DIB）技术在支原体简单鉴定和支原体抗体检测中的应用。在三种纤维素膜中，硬孔膜最适合用于这些用途，因为它比硝基纤维素或硝基加2000更具弹性和易于处理。由于交叉反应明显，完整的兔抗血清不足以用于支原体的鉴定，尽管同源抗血清的DIB滴度要高得多。完整SPF鸡血清含鸡毒杆菌（MG）和滑膜分枝杆菌（MS）抗原的DIB滴度较低，而经实验或自发感染MG和/或MS并含有阳性HI抗体的鸡血清具有较高的DIB滴度。结果表明，当检测血清被充分稀释时，DIB反应具有特异性。通过一定稀释技术进行的这项试验似乎可作为诊断禽支原体病的有用工具。

Application of the dot-immunobinding (DIB) technique to a simple identification of mycoplasmas and to the detection of mycoplasmal antibodies was investigated. Among three cellulose membranes, durapore membrane was revealed to be most suit-able for these purposes as it was more elastic and easy to handle than nitro-cellulose or Nitroplus 2000. Intact rabbit antisera were not sufficient for the identification of mycoplasmas by this technique due to the significant cross reactions, although homologous antisera resulted in much higher DIB titers.Sera from intact SPF chickens exhibited low DIB titers with M.gallisepticum (MG) and M.synoviae (MS) antigens, while sera from chickens experimentally or spontaneously infected with MG and/or MS and containing positive HI antibodies showed high DIB titers. It was suggested that DIB reaction was specific when test sera were sufficiently diluted. This test performed by a definite dilution technique seems to serve as a useful tool for diagnosing avian mycoplasmosis.