Studies on age-related changes in senescence accelerated (SAM-P) mice.

衰老加速小鼠（SAM-P）由日本京都大学Takeda教授建立。本研究关注SAM-P造血系统的年龄相关性变化。与年轻小鼠相比，老年小鼠的造血干细胞（CFU-S）和粒细胞-巨噬细胞集落形成细胞（GM-CFC）数量显著减少。然而，据报道，同一品系（SAM-R）的非加速小鼠也显示出与年龄相关的造血活性下降。这迫使我们使用其他品系的正常小鼠。BDFI和ddy株正常小鼠造血干细胞和GM-CFC数量均未见明显变化。肥大细胞祖细胞数量与年龄相关减少的可能性也在研究中，因为肥大细胞祖细胞似乎与CFU-S密切相关，而且我们已经建立了一个具有高播种效率的肥大细胞祖细胞体外检测系统，有助于对肥大细胞祖细胞进行定量评估。

Senescence accelerated mice (SAM-P) were established by Prof. T. Takeda at Kyoto University.This study concerns age-related changes in hemopoietic system of SAM-P.Numbers of hemopoietic stem cells (CFU-S) and granulocyte-macrophage colony-forming cells (GM-CFC) showed a significant decrease in old mice compared to those in young mice. Reportedly non-accelerated mice of the same strain SAM-R), however, also showed an age-related decrease in hemopoietic activity. This forced us to use normal mice of other strains.No significant changes were observed in normal mice of BDFI and ddy strains in the numbers of hemopoietic stem cells and GM-CFC.A possibility of age-related decrease in the number of mast cell-progenitors is also under study, since mast cell-progenitors appear to be closely related to CFU-S and since we have establishe an in vitro assay system of the progenitors with a high seeding efficiency which facilitates quantitative evaluation of mast cell progenitor.