Genetic and Biochemical Studies on H, Se, and X genes encoded fucosyltransferases responsible for biosynthesis of blood group antigens.

为了阐明-岩藻糖基转移酶与H、Se、Le和X1等基因的初级和次级产物岩藻糖基血型物质之间的关系，对参与合成岩藻糖基转移酶的-岩藻糖基转移酶进行了检测和鉴定。1)新的化学合成寡糖可用于确定糖基转移酶的特异性，为(1 2)-、(1 3)-、(1 4)-L岩藻糖基转移酶、N-乙酰-D-氨基葡萄糖基转移酶和唾液酸基转移酶提供了特异的检测系统。2)建立了用抗Le^a和抗Le^b的单抗测定人血清和唾液Lewis抗原的简便方法。应用这种免疫分析方法，在新生儿、孕妇和癌症患者的样本中证实了血型不合的Lewis抗原的表达。(1)--负责Le^a抗原的L-岩藻糖基转移酶

Detection and characterization of -fucosyltransferases responsible for the synthesis of fucoglycoconjugates in blood group substances were undertaken to elucidate the relationship between -fucosyltransferases and fucosyl blood group substances which were primary and secondary products of genes such as H, Se, Le and X.1) The availability of new chemically synthesized oligosaccharides for determining the specificities of glycosyltransferases could provide specific assay systems for (1 2)-, (1 3)-, (1 4)-L-fucosyltransferases, N-acetyl-D-glucosaminyltransferase and sialyltransferases. 2) A simple radioimmunoassy method was developed for the measurement of Lewis antigens in human sera and saliva with monoclonal anti-Le^a and anti-Le^b antibodies. Using this immunoassay method, the expression of incompatible lewis blood group antigens was demonstrated in new-born, pregnant samples and samples from cancer patients. (1 4)-L-Fucosyltransferase responsible for Le^a antigen