A study on intracellular energy status of the preserved liver by 31P-NMR spectroscopy

用31P-MMR光谱仪对小鼠保存肝细胞内高能磷酸盐进行了测定，分别在热缺血和冷缺血条件下进行。缺血后，肝脏用改良的Krebs-Henseleit缓冲液再灌流，并重新评估。ATP迅速下降，10分钟内检测不到。在热缺血和冷缺血条件下，当这些缺血保存的肝脏开始再灌流时，再次观察到ATP的合成。在保存时间小于30min的肝脏中进行ATP的再合成。在热缺血和10小时的情况下。以防出现冷缺血。肝切除前门静脉注射钙通道阻滞剂(维拉帕米)对再灌流时ATP合成减少有保护作用。肝脏MP/PI比值维持在较高水平，表明再灌流时ATP的恢复良好。OUR结果表明，在非灌流状态下，ATP对判断保存器官的存活率的价值有限。而MP/PI比率可用作可用于相同条件的指示器。

Intracellular high energy phosphate of the preserved mouse liver was measured by 31P-MMR spectrometer The measurements were done either under warm or cold ischemic condition. After ischimea, the liver was reperfused with modified Krebs-Henseleit buffer solution and reevaluted.ATP decreased rapidly and became undetectable within 10 min. under both warm and cold ischemia.Synthesis of ATP was observed again when reperfusion was started on these ischemically preserved liver. ATP was resynthesised in the liver preserved shorter than 30 min. in case of warm ischemia and 10 hr. in case of cold ischemia. Infusion of the Ca channel blocker(verapamil) in to the portal vein prior to hepatectomy was protective against a decrease in the synthesis of ATP at the time of reperfusion. The ratio of MP/Pi was maintained at high level in the liver which showed good recovery of ATP at the time of reperfusion.OUR results demonstrated the fact ATP has limited value on judgement of the viability of the preserved organ particulary under non-perfused state. While the MP/Pi ratio may be useful as an indicator available to the same condition.