Histological Study of the Granulation Tissue around The Loosened Prosthesis

对18例翻修全关节置换术标本进行了组织学研究。从骨水泥界面采集肉芽组织标本。肉芽组织中的细胞多为巨噬细胞样细胞，H2 E染色胞质较丰富。用抗巨噬细胞单抗(63D3)进一步研究表明，这些细胞为巨噬细胞来源。偏光显微镜下，部分细胞内可见大量高密度聚乙烯碎片。电子显微镜观察发现，这些细胞胞浆内有大量的空泡，其中含有溶酶体，提示这些细胞产生PGE_1和胶原酶等溶解细胞因子，对假体在骨水泥界面的松动起重要作用。

Eighteen specimens obtained at the time of revision sungery of total joint replacement were studied histologically. Specimens of the granulation tissue were collected from the bone-cement interface. Most of the cells observed in this granulation tissue seemed to be macrophage-like cells which had relatively abundant cytoplasm with H2 E staining. Further study utilized anti-macrophage monoclonal antibody (63D3) showed that these cells were macrophage origin. With polarized light microscopy, abundant fragments of high density polyethylene were found in some of these cells. Finally, electronmicroscopic observation revealed that these cells had numerous vacuoles which included lysosomes in their cytoplasm suggesting that these cells produced orteolytic cytokine such as PGE1 and collagenese and played important role for the loosening of the prosthesis at bone-cement interface.