Induction of differentiation on the differentiation of human embryonal carcinoma

为了建立人生殖细胞肿瘤细胞的体外分化体系，我们从9株来源于不同类型的人生殖细胞肿瘤的细胞系中初步筛选出一株细胞系NEC 14。从睾丸胚胎癌（EC）建立的NEC 14系在裸鼠异种移植瘤中自发地显示出多分化的能力，如腺成分、软骨组织和胚外合胞滋养细胞成分。作为体外分化诱导试剂，我们使用10 M的N，N ′-双乙酰胺（HMBA），因为该试剂在HMBA、视黄酸、孕酮、DMSO和dbc AMP中显示出最有效的诱导明显分化集落的活性。NEC 14细胞上的C抗原。此外，这些HMBA处理的NEC 14细胞显示出对塑料基底的更高粘附性，并且比未处理的NEC 14细胞含有更大量（3X）的纤溶酶原激活物。经过长时间的培养，角蛋白，结蛋白，和波形蛋白的hmba处理cells.In额外的项目，我们的特点是F9胚胎聚糖的分子特性，从生殖细胞肿瘤患者的血清中的一种独特的抗体识别的一个亚群。我们还发现，神经元特异性烯醇化酶（NSE）的测量是诊断价值，不仅为未成熟畸胎瘤，但也为无性细胞瘤。

To establish an in vitro differentiation system of human germ cell tumor cells, we initially selected a cell line, NEC 14 from 9 cell lines derived from various types of human germ cell tumors. The NEC 14 line established from testicular embryonal carcinoma ( EC ) showed spontaneously a capacity for multiple differentiation in xenograft tumors in nude mice, such as glandular components, cartilagious tissue, and an extraembryonic syncytiotrophoblastic element. As a differentiation inducing reagent in vitro, we used 10 M of N,N'- hexamethylene bisacetamide ( HMBA ), since this reagent showed the most potent activity to induce apparently differentiated colonies among HMBA, retinoic acid, progesterone, DMSO, and dbc AMP.FACS analyses revealed that the HMBA treatment reduced human EC antigen, SSEA-3, and inversely induced SSEA-1 and HLA-A, B, C antigen on NEC 14 cells. Furthermore, these HMBA-treated NEC 14 cells showed higher adhesivity to plastic substrate, and contained larger amount ( 3X ) of plasminogen activator than untreated NEC 14 cells. After prolonged culture, keratin, desmin, and vimentin were immunohistochemically identifiable on a subpopulation of these HMBA-treated cells.In additional projects, we characterized the molecular properties of F9 embryoglycan recognized by a unique antibody in sera from patients with germ cell tumors. We also found that neuron-specific enolase ( NSE ) measurements are of diagnostic value not only for immature teratomas but also for dysgerminomas.