An Experimental Study on the Biological Property of Periodontal Pocket-epithelium.

为阐明牙周袋上皮细胞的生物学特性，采用免疫组织化学、电子显微镜和示踪等方法。在大鼠上颌骨第一磨牙和第二磨牙之间插入弹性口香糖制成实验袋。在抗角蛋白抗体免疫组织化学标本中，囊袋上皮呈阳性染色，而正常连接上皮呈阴性。囊上皮和连接上皮的外基板均呈抗层粘连蛋白抗体阳性反应。电子显微镜观察，囊袋上皮呈多角形，胞质中央有一大核。细胞器不发达，粗面内质网和高尔基体较少，但有少量张力细丝。可以发现牙周袋形成的最初变化，即细胞到细胞连接的破坏，但不是牙齿表面上皮细胞的脱落。用过氧化物酶和镧可以检测到示踪剂穿过牙周袋上皮细胞间隙进入牙周袋。也就是说，袋上皮被提供了一些结构，允许一些物质通过细胞间隙。在上皮下方的结缔组织中可见丰富的毛细血管。相反，在与长连接上皮相对应的结缔组织中可检测到少量的毛细血管。这些结果提示：(1)袋上皮可能是低分化的角化细胞，(2)袋上皮具有允许某些物质通过细胞间隙的结构，因此，袋上皮具有与正常连接上皮相似的颈液保护功能，(3)检查牙侧是否有层粘连蛋白是确定连接上皮或袋上皮的有效方法。

To elucidate the biological property of periodontal pocket epithelium, immunohistochemistry, electron microscopy and tracer methods were employed. Experimental pocket was produced by insertion of elastic gums between the first and second molar of the rat maxilla. In specimens of immunohistochemistry using anti-keratin antibody, pocket epithelium showed positive staining, while normal junctional epithelium revealed negative. Outer basal lamina of both pocket and junctional epithelia showed positive reaction to anti-laminin antibody. In electron microscopic observation the pocket epithelium was polygonal in shape, and had a large nucleus located in the center of the cytoplasm. Poor-developed organelle, few rough endoplasmic reticulum and Golgi apparatus but a small amount of tonofilaments, was detected. Initial changes of periodontal pocket formation could be found, i.e., as a destruction of cell-to cell connection but not an exfoliation of epithelial cells from the tooth surface. It could be detected that tracers passed through intercellular spaces of the pocket epithelium into periodontal pocket in the present stuey using peroxidase and lanthanum. Namely, the pocket epithelium was provided structures allowing to pass some materiasl through tese intercellular spaces. Abundant capillaries were observed in the connective tissues just below the epithelium. On the contrary, few capillaries could be detected in the connective tissue corresponding to the long junctional epithelium. From these results, it is suggested that ; (1) pocket epithelium may be poorly differentiated keratinizing cell, (2) pocket epithelium has structures to allow passing some materials through their intercellular spaces, consequently, pocket epithelium is provided a protective function by cervical fluids in similar manner of normal junctional epithelium, (3) to examine the presence of laminin at the tooth side is effective to determine junctional epithelium or pocket epithelium.