Generation of fatty liver with volatile chloro-compounds in adult rat hepatocytes in primary monolayer culture.

用苯巴比妥预处理大鼠肝细胞并在甲吡酮存在下接种，细胞色素P-450的含量保持在与体内肝脏相当的水平，在原代单层培养的实验系统中研究了四氯化碳对细胞内三酰甘油周转率和酸性三酰甘油脂肪酶活性的影响。发现培养基中前体的浓度不影响三酰甘油的合成、分泌和水解的时间过程，四氯化碳显著抑制细胞内甘油三酯的水解和酸性脂肪酶的活性，并伴随甘油三酯的蓄积，提示四氯化碳可能是引起肝细胞内甘油三酯蓄积的原因之一四氯化物可能是通过抑制溶酶体酸性脂肪酶的分泌而介导的。

Pretreatment of rats with phenobarbital and plating the hepatocytes in the presence of metyrapone retained the content of cytochrome P-450 at the level equivalent to that in the liver in vivo.The effect of carbon tetrachloride on the turnover rate of the intracellular triacylglycerol and on the activity of acid triacylglycerol lipase was investigated in this experimental system of primary monolayer culture. It was found that the concentration of the precursors in the medium did not affect the time course of the synthesis, secretion, and hydrolysis of triacylglycerol, and that carbon tetrachloride significantly suppressed the hydrolysis of intracellular triacylglycerol and the activity of acid lipase with a concomitant accumulation of triacylglycerol.The result indicate a possibility that thetriacylglycerol accumulation in the cultured rat hepatocytes caused by carbon tetrachloride might be mediated by the inhibition of lysosomal acid lipase in addition to the suppression of the secretion.