Determination of ABO blood group of seminal stain mixed with vaginal secretion using immunoblotting

本研究从人精浆中分离出一种ABO血型糖蛋白，命名为α_2-葡糖蛋白（α_2-SGP）。用完全弗氏佐剂固定的α_2-SGP免疫家兔制备抗α_2-SGP血清，用SDS-PAG免疫印迹法检测精液和阴道液混合物中的ABO血型。(1)在阴道液中不能检测到α_2-SGP。提示α_2-SGP是精液ABO血型特异性糖蛋白。(2)结果表明，α_2-SGP的ABO血型活性与阴道液的ABO血型活性重叠，不能区分精液和阴道液的ABO血型，可作为精液ABO血型鉴定的一个有用的血型标志物。然而，这种方法被发现不适合用于法医学案件。

A purpose of this research is development of a new technique for the determination of each ABO blood group of semen and vaginal fluid in sexual cases.An ABO blood group glycoprotein, which was designated as "slpha_2-seminoglyco- protein (alpha_2-SGP)" was isolated from human seminal plasma. The anti-alpha_2-SGP sera were prepared by immunizing rabbits with the alpha_2-SGP emulsitied with complete Freund's adjuvant.A new method for determination of each ABO blood group of semen and vaginal fluid in mixture of both semen and vaginal fluid by SDS-PAG electrophoresis-Immunoblotting using anti-alpha_2-SGP sera, anti-A and anti-B was carried out.(1) The alpha_2-SGP cannot be detected in vaginal fluid. This result suggests that alpha_2-SGP is a seminal ABO blood group specific glycoprotein.(2) ABO blood group activity of alpha_2-SGP overlaps with that of vaginal fluid ; therefore each ABO blood group of semen and vaginal fluid could not be distinguished.Results show alpha_2-SGP to be a useful blood group marker for the determination of ABO blood group of semen. However, this method was found to be unsuitable for use with forensic science case.