Study on host factors in lung cancer patients

本研究的目的是检查肺癌患者和癌症高危人群中肿瘤的风险。1.苯并（a）芘（BP）对姐妹染色单体交换（SCE）的诱导作用：肺癌患者外周血淋巴细胞SCE频率显著高于正常人和低癌风险人群。暴露于BP后，肺癌患者和高癌症风险个体的淋巴细胞显示出比低风险个体显著更大的SCE产率。这一发现表明，高风险个体的淋巴细胞可能比低风险个体更容易受到BP诱导的DNA损伤。ras癌基因产物的表达：用MobAb rp 35检测肺肿瘤组织切片中ras p21的表达。原发灶直径大于30 mm的肿瘤材料与RP-35呈强阳性反应的频率显著高于原发灶直径小于或等于30 mm的肿瘤材料。阴性或可疑染色肿瘤患者的生存时间明显长于中度染色肿瘤或强染色肿瘤患者。这些结果提示ras癌基因可能在肺癌原发灶的生长或成瘤中起重要作用.微量营养素的家族因素：在家族成员中，尤其是腺癌患者的家族，血清硒和维生素E水平显著低于对照组。提示肺癌患者家族中血清硒和维生素E水平存在家族因素。

The purpose of this study is to examine the risk of neoplasma in lung cancer patients and high-cancer-risk individuals.1. The inducibility of sister chromatid exchange(SCEs) by benzo(a)pyrene(BP): The baseline SCE frequency in cultured lymphocytes was significantly high in lung cancer patients, as compared with all healthy persons or low-cancer-risk individuals. Following exposure to BP, the lymphocytes of lung-cancer patients and high-cancer-risk individuals exhibited significantly greater SCE yields than those of persons at low risk. This findings suggest that lymphocytes of high-risk individuals may be more susceptible to BP-induced DNA damage than those of persons at low risk.2. Expression of ras oncogene product: MobAb rp35 was used for detection of ras p21 in histologic section of resected lung tumors. Materials of tumors from primary sites larger than 30 mm in diameter exhibited intensely positive reactions with rp-35 significantly more frequently than those with primary sites, 30 mm in diameter or smaller. Patients with negatively or equivocally staining tumor had significantly longer survival times than did those with moderately staining tumor or those with intensely staining tumor. These results suggest that ras oncogene may play a significant role in growth or tumorigenesis at the primary site in lung cancer.3. Familial factors in micronutrients: Among Family members, particularly, families of adenocarcinoma patients, serum selenium and vitamin E levels were significantly lower than those in controls. These data suggest that there are familial factors in serum selenium and vitamin E levels among families of lung cancer patients.