STUDIES ON THE MECHANISM OF ANTIDEPRESSANTS USING CLONAL CELL LINES (II)

C6胶质瘤细胞在含有地塞帕明(DMI)或氟哌啶醇(HAL)的培养液中培养4d。DMI和HAL均能抑制异丙肾上腺素刺激的细胞内cAMP(CAMP)积聚。10 mU DMI可降低细胞总蛋白和细胞内ATP水平。DMI在细胞内的浓度大约是细胞外浓度的50倍。培养液中10 MU的DMI可能通过向细胞内蓄积而对C_6细胞产生毒性作用。因此，要研究DMI在C6细胞上的作用机制，其在培养液中的浓度应小于1um。C6细胞长期暴露于0.1-1um DMI，其剂量既不影响细胞总蛋白，也不影响细胞内ATP水平，但不能减少cAMP的积累。

C_6 gliom cells were cultured in the medium containing desipramine(DMI) or haloperidol(HAL) for 4 days. Not only DMI but also HAL, in a dose of 10 muM, reduced the accumulation of cyclic AMP (cAMP) in the cells stimulated by isoproterenol. 10 muM DMI reduced both total cell protein and ATP level in the cells. Intracellular level of DMI was approximately 50-fold more concentrated than the extracellular level. It is likely that 10 muM of DMI in the medium induce toxic effect on C_6 cells due to the accumulation into the cells. Therefore, to investigate the mechanism of action of DMI using C_6 cells, its concentration in the medium should be less than 1 muM. Chronic exposure of C_6 cells to 0.1-1 muM DMI, in which doses neither total cell protein nor ATP level in the cells was affected, did not decrease cAMP accumulation.