Correllation of Abnormal Production of Steroid Honnors by Adrenaltumors, wich Cytochroone p-450

用垂体切除大鼠，研究了胆固醇侧链裂解酶和21-羟化酶基因表达的改变，以及促肾上腺皮质激素增加c-fos和β-肌动蛋白基因表达。首先，诱导p-450 SCC mRNA的稳态水平比其他研究者在体外研究中观察到的更快。这些结果可能表明，在体内肾上腺的完整性是重要的促肾上腺皮质激素的行动。第二，促肾上腺皮质激素引起的c-fos和-actin基因表达的增加可能在调节促肾上腺皮质激素对肾上腺的作用中起重要作用。本文对两例特殊的醛固酮瘤患者与其他常见病例进行了对比研究。在两组中，编码类固醇生成酶的mRNA不仅存在于非肿瘤组织中，而且存在于肿瘤组织中。定量分析结果表明，在地塞米松抑制试验（DST）阳性的肾上腺组织中，非肿瘤组织中的mRNA含量高于肿瘤组织。然而，在DST（-）特别是两个病例中，肿瘤组织中的mRNA含量要高得多。这些结果表明，DST（-）患者的肿瘤细胞不仅能自主合成醛固酮，而且还能自主合成皮质醇。

With hypophysectomized rat, alteration in the expression of genes for cholesterol sidechain cleavage enzyme and 21-hydroxylase, and adrenocorticotropin increases expression of c-fos and beta -actin genes were investigated. AT first, the induction of steady-state levels of p-450scc mRNA was faster than that observed by other investigators in studies in vitro. These results may indicate that integrity of the adrenal gland in vivo is important for the action of ACTH. At the second, increased expression of c-fos and -actin genes by ACTH may have important roles in mediating its action on adrenals. Two particular patients with aldosterone-producing tumors were studied in companison with other usual cases. In both groups, mRNAs coding steroidgeneic enzymes were present not only in the non-neoplastic but also in the tumor tissues. Quantitative analyses of the mRNA levels revealed that in the adrenals from dexamethasone suppression test (DST) (+) patients, the mRNAs were more abundast in non-neoplastic tissues than in tumor tissues. However, in those from DST (-) particular two cases, the mRNAs were much more abundant in the tumor tissues. These results indicate that tumor cells of the DST(-) patients autonormously synthesized not only aldosterone but also cortisol.