Chemosensitivity of Ovarian clear Cell Carcinoma Revealed by Disease Oriented Screening System.

采用体外MTT法研究卵巢透明细胞癌的化疗敏感性。本研究采用四种已建立的人卵巢透明细胞癌细胞系：HAC-2、OVAS、RMG-I和KK。检测了ADM、THP-ADM、EPI、ACR、DM、PEP、BLM、Act-D、MMC、NCS、VLB、VCR、VDS、CPM、MTX、ala - c、5-FU、L-ASP、CDDP、254-S、CBDCA、NCNU、ETP等23种抗肿瘤药物。将细胞以1或3 × 10^4/孔（第0天）接种于微孔板，培养3天，分别以0、0.01、0.05、0.1、0.2、0.5、1、2、5、10 mic g/ml的浓度暴露于每种药物中，分别暴露于1、3、6、12、24、48、72h（第3天），洗净游离药物，单独培养培养基。MTT法测定酶活性（第7天）。通过存活分数曲线评价各药物的抗肿瘤作用。本研究结果显示RMG-I具有多药耐药，且所有药物均无效。其他3种细胞系的药物敏感性相似，对该肿瘤最有效的药物为Act-D、CDDP和254-S。

Studies were undertaken to examine the chemosensitivity of ovarian clear cell carcinoma in vitro by MTT assay.Four kinds of established human ovarian clear cell carcinoma cell line, HAC-2, OVAS, RMG-I and KK were used in this study.Twenty-three kinds of antitumor agents including ADM, THP-ADM, EPI, ACR, DM, PEP, BLM, Act-D, MMC, NCS, VLB, VCR, VDS, CPM, MTX, Ara-C, 5-FU, L-ASP, CDDP, 254-S, CBDCA, NCNU, ETP were examined.The cells were inoculated into microplate at 1 or 3x10^4/well (day 0), cultured for 3 days and exposed to each drug at 0, 0.01, 0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10 mic g/ml, for 1, 3, 6, 12, 24, 48, 72hs (day 3), washed free drug and incubate media alone. The enzyme activities were measured by MTT assay (day 7). The antitumor effects of each drug were evaluated from the surviving fraction curves.As a result of this study, RMG-I had multidrug resistance and none of drugs were effective. Other 3 kinds of cell lines have similar drug sensitivities and the most effective drugs on this tumor were Act-D, CDDP and 254-S.