Androgen matabolism in normal and malignant laryngeal tissue

以日本白羊的喉、小鼠和人的喉部组织为研究对象，检测这些组织是否为雄激素的靶点之一。通过^ 3h -睾酮、放射自显影、体内注射研究或组织培养等方法，我们认为正常和恶性喉部组织，尤其是喉部组织上皮是雄激素的靶器官或靶组织之一。此外，在细胞核中观察到氧化银颗粒显示放射性标记的类固醇，这与在众所周知的类固醇靶组织中观察到的现象相同。我们的免疫组化研究显示8例喉癌标本中有2例抗雄激素抗体阳性细胞。这些现象提示安多素在喉部正常发育中起重要作用，也是喉癌发生和/或生长的致癌因子或辅助因子之一。1 / 7的喉癌可以植入小鼠模型，但该菌株对雄激素和其他类固醇没有反应。我们正在研究喉癌中雄激素受体mRNA的定向表达，已经检测了5例喉癌细胞中雄激素受体的产生，但尚未成功。

Sylynx of Hen (White Japanese reghon), larngeal tissue of mice and human were used and studied to detect whether these tissues are one of targets for androgen. By utilizing ^3H-testosterone, autoradiography and in vivo injection study or tissue culture method, we concluded that noramal and malignant laryngeal tissue, especially epithelium of these tissues, are one of target organs or tissues for androgen. Farthermore, oxidized silver grains demonstrating radio labeled steroid was observed in the nucleus of cells as the same phenomena as obserbed in well known steroid target tissues.Our immunohistochemical studies demonstrated anti-androgen antibody Positive cell in two out of 8 laryngeal carcinoma specimens. These phenoena implies that andogrn plays an important role in normal laryngeal development and as one of carcinogenic factors or cofactors in laryngeal carcinoma development and/or growth.One out of 7 laryngeal carcinomas could be planted mude mice, and however this strain did not show reactivity to androgen and other steroids.We are studying to ditect mRNA of androgen recepter in laryngeal carcinoma, 5 samples have been examined to determine production of androgen recepter in laryngeal carcinoma cell and have not succeeded in this point yet.