Patch-clamp studies on the membrane properties of granulocytes and their changes during cell-activation.

从蝾螈(Triturus Pyrrhogaster)的心房中获取的血液中含有几种类型的白细胞。相差显微镜下可见三种类型的粒细胞。使用贴片电极，可以研究这些细胞的全细胞膜电流。在中性粒细胞(直径19-23微米)上，从-83 mV的保持电位以超极化方式诱发内向整流钾电流。在外加Cs~+(1 MM)或Ba~(2+)~(2+)(1 MM)时，内向电流的幅度随时间和电压的变化而减小。当外加K~(++)浓度从4 mM到20 mM增加到100 mM时，K~(++)电流的阈值从-83 mV到-43 mV移动到-3 mV。随着外加K~(++)浓度的平方根增大，诱发的K~(++)电流的幅度也随之增大。它们的I-V关系几乎是线性的，这表明这些细胞几乎具有电不可激发的性质。比较了粒细胞和内分泌细胞的这些电生理特性。

The blood obtained from the atria of the newts (Triturus pyrrhogaster) contains several types of leukocytes. Under a phase contrast microscope, three types of granulocytes could be identified. Using patch-electrodes, it was possible to investigate the whole-cell membrane currents in these cells. In the neutrophils (diameter, 19-23 mum), identified by characteristic features of lobulated nucleus, inward rectifying K^+ currents were evoked with hyperpolarization from the holding potential of -83 mV. In the presence of Cs^+ (1 mM) or Ba^<2+> (1 mM) in the external saline, the amplitudes of inward currents were reduced with time- and voltage- dependent manner. When the external concentration of K^+ was increased from 4 mM through 20 mM to 100 mM replacing isomolar choline in the perfusing salines, the threshold of the K^+ currents shifted from -83 mV through -43 mV to -3 mV.The amplitude of the evoked K^+ currents also increased as the chord conductance in each of these salines was nearly proportional to the square root of the external K^+ concentration.In basophils and eosinophils, there evoked no major membrane currents under voltage-clamp conditions. Their I-V relations were almost linear, which suggested almost electrically non-excitable properties in these cells. These electrophysiological properties of granulocytes were compared with those of endocrine cells.