Locomotor Changes of Cat Forelimb and CNS-Metabolic Changes using Cytochrome oxidase Histochemistry and 2-DG After Destrction of Medullary Pyramis or Cervical Dorsal Roots

运动恢复尚未完全了解，因此我们使用细胞色素氧化酶组织化学（Wong-Riley，1976）和14 C-脱氧葡萄糖放射自显影（Sokoloff等人）研究了延髓切断猫的运动障碍和代谢变化。1077）。运动功能被检查为进食运动（Gorska等人，1980年）和体检。术后1周对4只猫进行细胞色素氧化酶组织化学染色，术后7周对2只猫进行细胞色素氧化酶组织化学染色。所有的猫都有正常的休息姿势和姿势，但不能从圆筒内取出食物。所有猫均表现出对侧屈肌易化和伸肌抑制。组织化学显示所有猫均无偏侧性。4只猫术后1 wk进行14 C脱氧葡萄糖放射自显影，发现同侧丘脑腹外侧核的局部脑葡萄糖代谢率降低。然后用WGA-HRP法研究了经颈前根与一侧肌肉有关的中枢神经系统。切断C4 ~ C8后根，将WGA-HRP植入周围神经。手术后两天和一周，将猫处死进行组织学检查。双侧前角细胞、后角细胞、背柱核、下橄榄核、小脑核、斜方体、红核、黑质、丘脑核和内囊均有反应。这么多的网站显示了一种可能性有关的运动恢复。

Motor recovery is not fully understood, so we studied motor disturbance and metabolic changes in medullary pyramidotomized cats using cytochrome oxidase histochemistry(Wong-Riley, 1 976)and 14C-deoxiglucose autoradiography(Sokoloff et a]., 1077). Motor function was examined as food-taking movement(Gorska et al., 1 980)and physical examination. Cytochrome oxidase histochemistry was done to 4 cats I wk after the surgery and to 2 cats 7 wks after the surgery. All cats had normal resting posture and ambulation, but could not retrieve food from within a cylinder. The all cats showed contralateral flexor facilitation and extensor inhibition. The histochemistry showed no laterality on the all cats. 14Cdeoxiglucose autoradiography I wk after the surgery was done for 4 cats, showed that the local cerebral metabolic rate for glucose decreased in the ipsilateral thalamic VPL nucleus. Then we studied CNS concerning to one muscle via cervical ventral roots using WGA-HRP. The dorsal roots C4 to C8 were cut and WGA-HRP was implanted to the peripheral nerve. Two days and I wk after the surgery, cats were sacrificed to histology. Bilateral anterior horn cells, posterior horn cells, dorsal column nuclei, inferior olives, cerebellar nuclei, trapezoid bodies, red nuclei, substantia nigras, thalamic nuclei, and internal capsules were reacted. These so many sites showed one possibility concerning to the motor recovery.