Immunohistochemical study of ameloblastoma

应用抗广谱角蛋白（TTL）的多克隆抗体和抗低分子量角蛋白（PKK 1）和高分子量角蛋白（KL 1）的单克隆抗体，对27例成釉细胞瘤角蛋白表达进行了免疫组化研究，以阐明肿瘤的分化。其中丛状型15例，滤泡型12例，棘皮瘤型2例，基底细胞型3例。（三个病例在每个肿瘤中包含两种或两种以上组织学类型的区域，并在每个类别中计数。滤泡状和丛状成釉细胞瘤中柱状细胞和星状细胞角蛋白阳性率较高。滤泡型成釉细胞瘤中，星状细胞TTL或KL 1的阳性率高于PKKI（P<0.05）;丛状型成釉细胞瘤中，柱状细胞PKK 1的阳性率高于KL 1（P<0.05）。滤泡型星形细胞TTL或KL 1染色阳性率显著高于丛状型（p<0.025）。与TTL和KL 1抗体的反应通常在滤泡性或棘皮瘤性成釉细胞瘤的星状细胞中呈阳性。基底细胞型成釉细胞瘤细胞巢PKK 1阳性，提示滤泡型成釉细胞瘤和棘皮瘤型成釉细胞瘤易于向鳞状细胞分化，而丛状型成釉细胞瘤仍处于肿瘤分化的原始阶段。

Keratin expression in 27 ameloblastomas was studied immunohistochemically in order to clarify the tumor differentiation using polyclonal antibody against wide spectrum keratins ( TTL ), and monoclonal antibodies against lower and higher molecular weight keratins ( PKKl and KLl respectively ). These materials comprised 15 cases of plexiform type, 12 cases of follicular type, two cases of acanthomatous type, and three cases of basal cell type. ( Three cases contained areas with two or more histologic types in each tumor and were counted in each category.)Positive frequency of keratins in columnar cells and stellate cells were examined in the follicular and plexiform ameloblastomas. In the follicular ameloblastoma, the stellate cells were more frequently stained with TTL or KLl than with PKKI ( P<0.05 PKKl was more frequently positive in the columnar cells of the plexiform ameloblastoma than KLl ( p<0.05 ). Positive frequency of TTL or KLl staining in the stellate cells was significantly higher in the follicular type than in the plexiform type ( p<0.025 ). Reactions with TTL and KLl antibodies were generally positive in the stellate cells of the follicular or acanthomatous ameloblastomas. Cell nests of the basal cell type wore positive for PKKl.From these facts, it is concluded that the follicular ameleblastomn as well as acanthomatous one is liable to undergo squamous differentiation whereas the plexiform amoloblastoma remains in primitive stage of tumor differentiation.