Role for membrane phospholipids in cell-cell interaction

我们从血小板和肥大细胞中纯化磷脂酶A2，并研究了其激活机制（K. Inoue）。我们还从大鼠肠黏膜中纯化了磷脂酶A2的几个同工酶，并阐明了它们在不同细胞类型中的分布（M. Okamoto）。我们发现膜磷脂的过氧化导致血小板中磷脂酶A2的激活（T. Fujii）。我们发现产生二酰基甘油（DG）的能力与淋巴毒素的抗性密切相关，并阐明了淋巴毒素的细胞毒性作用引起磷脂酶A2活化的机制（T. Osawa， S. Toyoshima）。我们纯化并鉴定了人血小板中的磷脂酶C （Y. Nozawa）。我们证实了磷脂酶C在大鼠肝核部分的存在，并在细胞增殖过程中发现了不同分子种类的酶的诱导作用。我们在中性粒细胞和肺泡巨噬细胞中发现了血小板活化因子（PAF）产生的调节因子（K. ... More Waku），我们在未受刺激的组织如胃、心脏和子宫中发现了PAF的发生（K. Saito）。我们建立了一种使用paf特异性抗体（S. Nojima）的放射免疫测定方法。我们发现患者缺乏PAF乙酰水解酶，并从他们的血清中纯化了PAF释放因子（Y. Sizuki）。我们阐明了胶原活化血小板的机制（M. Kito）。我们从大鼠脑中纯化了与细胞内信号转导有关的磷脂酰肌醇（PI）激酶（T. Takenawa）。我们研究了pi锚定蛋白（H. Ikezawa）的功能。我们从大鼠肝脏中纯化了DG脂肪酶，并研究了其激活机制（Y. Nakazawa）。我们还研究了影响磷脂酰丝氨酸生物合成的遗传因素以及鞘脂在细胞增殖中的作用（Y. Akamats）。我们研究了脂肪酸分子种类对抗体和肿瘤坏死因子产生的影响（H. Okuyama）。我们研究了脂肪酸的理化性质（A. Hamada）。我们化学合成了非天然磷脂，并研究了它们在生物膜中的功能（T. Muramatsu）。少

We purified phospholipase A2 from platelets and mast cells and studied a mechanism for its activation (K. Inoue). We also purified several isozymes of phospholipase A2 from rat intestinal mucosa and clarified their distribution among different cell types (M. Okamoto). We found that peroxidation of membrane phospholipids leads to the activation of phospholipase A2 in platelets (T. Fujii). We showed that an ability to produce diacylglycerol (DG) is closely related to resistibility to lymphotoxins and clarified a mechanism for phospholipase A2 activation caused by cytotoxic action of lymphotoxins (T. Osawa, S. Toyoshima). We purified and characterized phospholipase C in human platelets (Y. Nozawa). We demonstrated the occurrence of phospholipase C in rat liver nuclear fraction and found an induction of different molecular species of the enzyme during cell proliferation.We found a regulatory factor for platelet-activating factor (PAF) production in neutrophils and alveolar macrophages (K. … More Waku), We found occurrence of PAF in unstimulated tissues such as stomach, heart and uterus (K. Saito). We established a radioimmunoassay method employing PAF-specific antibodies (S. Nojima). We found patients deficient in PAF acetylhydrolase and purified a PAF-releasing factor from their sera (Y. Sizuki).We clarified a mechanism for platelet activation by collagen (M. Kito). We purified phosphatidylinositol (PI) kinase concerning intracellular signal transduction from rat brain (T. Takenawa). We studied the function of PI-anchoring protein (H. Ikezawa). We purified DG lipase from rat liver and studied a mechanism for its activation (Y. Nakazawa). We also studied genetic factors which affect phosphatidylserine biosynthesis and a role for sphingolipid in cell proliferation (Y. Akamats). We studied effects of fatty acid molecular species on production of antibodies and tumor necrosis factor (H. Okuyama). We studied physicochemical properties of fatty acids (A. Hamada). We chemically synthesized unnatural phospholipids and studied their function in biological membranes (T. Muramatsu). Less