Phytochrome-mediated formation of terpenoids and glandular trichomes, and rapid tyrosine phosphorylation of a 25-kDa protein in thyme seedlings

生长4天的百里香幼苗经2.0Wm~(-2)红光照射30min后，子叶中单萜、倍半萜、三萜和四萜的含量显著增加。这种光反应在红光和远红光交替照射30min时是可逆的，表明光敏色素参与了各种萜类化合物的光调节。这种光可逆性在子叶中形成白色毛状体的情况下也观察到，这是单萜烯的生物合成部位。我们最近发现，R/FR光可逆性可以刺激25000-MR蛋白的甲状腺快速磷酸化，提示酪氨酸激酶(S)的激活可能参与了光敏色素的信号转导。

The exposure of dark-grown, 4-day-old seedlings of thyme(Thymus vulgaris L., Labiatae)to red light(2.0 Wm^<-2>)for 30 min caused a significant increase in the levels of mono, sesqui-, tri- and tetraterpenoids in cotyledons. This photoresponse proved to be reversible by alternate 30-min periods of irradiation with red and far-red(2.0 Wm^<-2>)light, indicating the participation of phytochrome in the photoregulation of the synthesis of various terpenoids. Such photoreversibility was also observed in the case of the formation of blandular trichomes, biosynthetic sites of monoterepenes, in the cotyledons. We recently found that R/FR photoreversibility was operative in the stimulation of rapid thyrosine phosphorylation of a 25000-Mr protein, suggesting that the activation of tyrosine kinase(s)could be involved in the signal transduction of phytochrome.