Synthesis of Mucus Glycoprotein in Ribosome of Rat Gastric Mucous Cell.

用胃粘膜细胞合成分泌性O-糖甙糖蛋白（粘蛋白），研究了蛋白质核心合成与其与脂肪酸酰化和与N-乙酰半乳糖胺O-糖基化的关系。用[^3H]-脯氨酸、[^3H]-棕榈酸、[^3C<14>]-半乳糖胺标记细胞，分离出肽段，并与相应的蛋白质核心进行了比较。tRNA和新生肽的释放表明大鼠胃粘膜细胞核糖体中的新生肽已经糖基化和酰化.前列腺素E-1对酒精性胃粘膜损伤的预防作用研究了乙醇和前列腺素对胃粘液细胞糖蛋白合成的影响。大鼠胃粘膜细胞在乙醇和前列腺素E1存在下，使用[^3H]-脯氨酸、[^3H]-棕榈酸、[<14>^3C]-半乳糖胺进行短期组织培养。低浓度乙醇（0.05-0.1M）刺激糖蛋白合成， ...更多信息 高浓度乙醇（0.5-1.5M）使糖蛋白合成明显减少。前列腺素的加入使乙醇存在下粘液糖蛋白的合成和分泌稳定.浸水应激对大鼠胃黏膜粘液糖蛋白合成的影响浸水应激大鼠胃黏膜细胞与[^3 H]-棕榈酸和[^<14>C]-N-乙酰半乳糖胺孵育。用冷乙醇沉淀胃多聚体释放的肽基-tRNA，然后测定其含量。与对照大鼠相比，在应激大鼠中观察到分离的肽基tRNA减少70%。从肽酰-tRNA回收的肽显示应激大鼠的[~ 3 H]-棕榈酸和[~ <14>1C]-N-乙酰半乳糖胺掺入比正常对照低30-50%。提示应激对胃粘膜核糖体多肽的翻译、酰化和糖基化有显著影响。少

Using gastric mucous cells which are involved exclusively in the synthesis of secretory O-glycosidic glycoprotein (mucin), the relationships between protein core synthesis and its acylation with fatty acids, O-glycosylation with N-acetylgalactosamine were investigated Labeling of the cells with[^3H]-proline, [^3H]-palmitic acid, [^<14>C]-galactosamine followed by isolation of peptidy -tRNA and release of nascent peptides indicated that the nascent peptide were already glycosylated and acylated in the ribosome of the rat gastric mucous cell.1. Prevention of alcohol-induced gastric mucosal injury by prostaglandin E-1 Effects of ethanol and prostaglandin on the glycoprotein synthesis in the gastric mucus cells have been evaluated. The rat gastric mucosal cells were subjected to a short-term tissue culture in the presence of ehtanol and prostaglandin El using[^3H]-proline, [^3H]-Palmitic acid, [^<14>C]-galactosamine. Low concentration of ehtanol (0.05-0.1M) stimulated the glycoprotein synt … More hesis, but higher concentration of ehtanol (0.5-1.5M) caused a marked reduction in the glycoprotein synthesis. Addition of prostaglandin resulted in the stabilization of the synthesis and secretion of the mucucs glycoprotein in the presence of ethanol.2. lnfluence of water-immersion stress on synthesis of mucus glycoprotein in rat gastric mucosa.Gastric raucous cells of rats subjected to water immersion stress were incubated with[^3H]-palmitic acid and[^<14>C]-N-acetylgalactosamine. The peptdyl-tRNA released by gastric polysomes was precipitated with cold ethanol and then the content was determined. A 70% reduction in the peptidyltRNA isolated was observed in the stress rats, as compared with in control rat. The peptide recoverd from the peptidyl-tRNA showed 30-50% less[3H]-Palmitic acid and[^<14>C]- N-acetylgalactosamine incorporation in the stressed rats than in normal control. It was thus suggested that translation, acylation and glycosylation of the peptides in the ribosomes of the gastric mucosa were remarkably affected by the stress. Less