Research of Ovum Capture Inhibitor (OCI) in Endometriosis ; a Study of Anti-OCI Production, Exploitation of Immunological OCI Measurement, Localization and Production of OCI.

我们在子宫内膜异位症腹膜液（PF）中发现了丰富的OCI（卵子捕获抑制剂）。OCI在金仓鼠毛上生成膜（OCIRM: OCI相关膜），体外抑制卵丘拾取。OCIRM由纤维状和碎屑状部分组成。我们设计这些研究是为了了解OCI的特性。1)评价OCI分子量，研究了PF （Sephacryl S-300）凝胶过滤的OCI分子量。在20 - 600K道尔顿滤液聚集处发现了OCI活性。2)用抗纤维蛋白原和抗PF对OCIRM进行免疫染色（PF<PF免疫兔血清用微孔过滤器>过滤超过1OOK道尔顿），研究两种药物对OCIRM的抗oci活性。纤维部分均有染色，但未见债样部分染色。两种药物均未抑制OCI活性，但仅产生类似债务的部分。3)用3种凝集素（Con-Al， WGA， SBA）进行免疫染色研究。4)研究了PF的OCI活性与总蛋白和纤维蛋白原浓度的关系，以及PF与凝集素（Con-A、WGA、Lentil）沉积量的关系（N=13）。OCI活性与总蛋白和纤维蛋白原浓度、沉积物量与凝集素（除扁豆）无关。结论：OCI由多个大分子（200- 600K道尔顿）组成。OCIRM纤维状部分疑为纤维蛋白，碎片状部分疑为凝集素结合糖缀合物。OCI活性与纤维蛋白原和凝集素结合物质一起出现，但纤维蛋白原不是必需的，主要因素是凝集素结合物质。

We have found out the OCI (ovum capture inhibitor) abundant in endometriosis peritoneal fluid (PF). OCI makes membranes (OCIRM : OCI related membranes) at Golden hamster fimbria which inhibit oocyte-cumulus pick up in vitro. OCIRM constitutes of fibrous and debris like part. We designed these studies to find out the characterization of OCI.1) To evaluate the OCI molecular weight gel filtration of PF (Sephacryl S-300) was studied. OCI activity was found at 200- 600K Daltons filtrates gatherings.2) Immuno-stain of OCIRM by anti-fibrinogen and anti-PF (immunized rabbit serum by PF<PF was filtrated over 1OOK Daltons with mi11ipore fi1ter>), and anti -OCI activity with both agents were studied. Fibrous part was stained by both but not debtis like part. OCI activity was not suppressed by both agent, but debtis like part was only made.3) With 3 Lectins (Con-Al WGA, SBA) immuno-stainings were studied. OCIRM were stained by Con-A and WGA.4) OCI activity of PF versus total protein and fibrinogen concentration, sediment amount of PF (PF with lectin makes sediments) with lectins (Con-A, WGA, Lentil) were studied (N=13). No relationships were noted between OCI activity and total protein and fibrinogen concentrations, sediment amount with lectins other than Lentil. Conclusions : OCI is constituted of plural large molecules (200- 600K Daltons). Fibrous part of OCIRM was fibrin suspected, but debris like part was lectin binding glycoconjugates suspect. OCI activity appears with fibrinogen and lectin binding substances, but fibrinogen is not necessary and major factor is lectin binding substances.