Improvement and application of high-vacuum evaporater with condensed-light beam heater for deep-etching replication of TEM specimen

本项目提出并完成了JFD-9010S(JOEL)型高真空蒸发器。它的特点是带有涡轮分子和低温吸收泵的清洁真空，与传统的油扩散泵不同。为了便于在低温阶段保持的断口表面进行深度刻蚀或升华过程，采用了聚光加热装置。利用这种创新的设备，在40-60分钟的照射后，样品被保持在-110到-120ﾟC，可以获得足够的深度刻蚀，将升华限制在要复制的非常表面。生物膜结构，包括光感受器细胞、嗜盐细菌和糖尿病神经纤维，正在进行应用。最后的数据将用专门的软件对投射到CRT上的透射电子显微镜图像上的膜内颗粒和其他深蚀刻精细结构进行定量分析。

A high-vacuum evaporater has been proposed and completed in the form of JFD-9010S (JOEL) by this project. It is characterized by 'clean vecuum' with a turbomolecular and a cryo-absorption pump, keeping out the traditional oil-diffusion pump. For facilitating the deep-etching or sublimation process on the fractered surface of the specimen maintained at the low-temperature stage, a condensed-light heating device has been adopted. Using this innovated device, adequate deep-etching is obtained after 40-60 min irradiation during which the specimen is maintained at -110 to -120ﾟC, limiting the sublimation to the very surface to be replicated.Application to the biological membrane structure, including of photoreceptor cells, halobacteria, and diabetic neural fibers, is going on. The final data will be quatitatively analyzed with the special soft-ware arranged for counting the intramembrane particles and other deep-etching fine structures on TEM pictures projected to CRT.