Preparation of adsorbents having a high affinity for endotoxins with polyamino acid

研究了对百日咳杆菌培养上清液中的内毒素具有高亲和力的吸附剂的制备。以聚谷氨酸甲酯(PMLG)为基质，氨基为配体时，即使在较高的离子强度(mU=0.2~0.4)下，对内毒素的亲和力也最高。PMLG珠子。含约3.2meq/g氨基的百日咳杆菌培养上清液可选择性去除内毒素，而不影响保护性抗原。结果表明，1ml的湿式吸附剂可吸附4.5 mg的内毒素。因此，PMLG衍生物的微珠被认为是去除百日咳疫苗中的内毒素的有效吸附剂，对丝状血凝素和百日咳毒素都没有影响。

Preparations of adsorbents having a high affinity for endotoxins in the culture supernatant fluids of Bordetella pertussis were investigated. When poly (gamma-methyl L-glutamate) (PMLG) was used as a matrix and amino groups was used as the ligand, the highest affinity for endotoxin was attained even at high ionic strength (mu=0.2-0.4). PMLG beads. containing amino groups of about 3.2 meq/g selectively removed endotoxin in culture supernatant of B. pertussis without affecting the protective antigens. It was demonstrated that 1 ml of the wet adsorbent adsorbed 4.5 mg of endotoxin. The beads of PMLG derivatives, therefore, are considered to be useful adsorbent for the removal of endotoxin from pertussis vaccine affecting neither filamentous hemagglutinin nor pertussis toxin.