Activation mechanism of lectin-induced response of lymphocytes by polycationic and polyanionic compounds.

众所周知，淋巴细胞的表面电荷对其丝裂原诱导的转化具有重要意义。这表明，用神经氨酸酶治疗大鼠淋巴结淋巴细胞或添加聚阳离子，聚l -鸟氨酸或聚d -赖氨酸，增强了它们对分裂原的反应。先前工作者的其他实验表明，在正常血清中发现的一些糖蛋白干扰了淋巴细胞对有丝分裂原的体外反应。这些糖蛋白如α -糖蛋白的一个特点是唾液酸含量相对较高。Larsen和Heron报道了人类淋巴细胞的丝裂原诱导反应被聚d -赖氨酸、deae -葡聚糖、硫酸维生素和甲基化血清白蛋白等多阳离子化合物增强，而被多阴离子化合物、铜蓝蛋白和硫酸软骨素抑制。我报道了硫酸软骨素A对豆豆蛋白A诱导的淋巴细胞有丝分裂的影响。低浓度硫酸软骨素增强了次优剂量的豆豆蛋白A诱导淋巴细胞的反应。硫酸软骨素增加了刀豆蛋白a诱导的淋巴细胞有丝分裂中^ 3h -胸腺嘧啶的掺入和母细胞的数量。在刀豆蛋白a缺失的情况下，硫酸软骨素增加了淋巴细胞的活力和细胞表面电荷，但对刀豆蛋白a诱导的Ca^<2+>流入淋巴细胞没有影响。

It is known that the surface charge of lymphocytes is of importance for their mitogen-induced transformation. This was suggested from the fact that the treatment of rat lymph-node lymphocytes with neuraminidase or the addition of polycations, poly -L-ornithine or poly-D-lysine, enhanced their response to mitogens. Other experiments by previous workers showed that some glycoproteins found in normal serum interfered with the in vitro response of lymphocytes to mitogens. One characteristic of these glycoproteins such as alpha-glycoproteins is a relatively high sialic acid content. Larsen and Heron reported that the mitogen-induced response of human lymphocytes was enhanced by such polycationic compounds as poly-D-lysine, DEAE-dextran, protamin sulfate and methylated serum albumin, whereas it was inhibited by the polyanionic compounds, ceruloplasmin and chondroitin sulfate. I report the effects of chondroitin sulfate A on the lymphocyte mitogenesis induced by concanavalin A. A low concentration of chondroitin sulfate enhanced a suboptimal dose of concanavalin A -induced response of lymphocytes. Chondroitin sulfate increased the incorporation of ^3H-thymidine and the number of blast cells in lymphocyte mitogenesis induced by a suboptimal dose of concanavalin A. Chondroitin sulfate increased the viability and the cell surface charge of lymphocytes in the absence of concanavalin A, but it did not affect the concanavalin A -induced Ca^<2+> influx into lymphocytes.