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Lymphocyte Transformation Test is unreliable for the diagnosis of kampo-medicine-induced liver injury.

淋巴细胞转化试验对于汉方药引起的肝损伤的诊断并不可靠。

基本信息

批准号：
15590596
负责人：
MANTANI Naoki
金额：
$ 0.51万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590596/
关键词：
drug-induced liver injury Kampo Medicine Lymphocyte Transformation Test false-positive

项目摘要

To examine actual impact of mitogenic activity by Kampo medicine on the lymphocyte transformation test(LTT), we conducted LTT for four herbs in patients who had taken no Kampo medicine in their life and consulted our department for the first time. Fourteen patients were enrolled for Toki (Angelicae Radix) and Ogon (Scutellariae Radix), and 4 patients were enrolled for Saiko (Bupleuri Radix) and Kanzo (Glycyrrhizae Radix). Patients who were being treated with corticosteroids or immunosuppresive drugs or who had experienced drug allergy were excluded. Each herb (5g) was added to 600 ml of water and boiled for 30 min. The boiled-down extract was submitted to Bio Medical Laboratories (BML, Inc.Japan), along with blood samples obtained from the enrolled patients. To examine the effects of herbs under the actual conditions of the LTT in the clinical situation, measurement of LTTs were performed as described previously ^<2)>by BML, Inc.LTT for both Toki and Ogon were positive in 12 of 14 patients. LTT for Saiko was positive in all 4 patients and LTT for Kanzo was positive in 3 of 4 patients. No eosinophilia or liver injury was observed in the patients. In the present study, we conducted LTT for these herbs in patients who had taken no Kampo medicine in their life. These findings suggests that LTT for the herbs are likely to yield false-positive results and LTT is unreliable for the diagnosis of Kampo-medicine-induced liver injury.

为了检查汉方药物的促有丝分裂活性对淋巴细胞转化试验（LTT）的实际影响，我们对生活中没有服用汉方药物并首次咨询我科的患者进行了四种草药的LTT。14例患者入组Toki（葛根）和Ogon（葛根），4例患者入组Saiko（柴胡）和Kanzo（葛根）。排除了正在接受皮质类固醇或免疫抑制药物治疗或发生药物过敏的患者。将每种草药（5 g）加入600 ml水中煮沸30 min。将煮沸的提取物与从入选患者中获得的血样一起沿着提交给Bio Medical Laboratories（BML，Inc.Japan）。为了检查草药在临床情况下LTT的实际条件下的效果，如前所述由BML，Inc.进行LTT的测量。14名患者中有12名Toki和Ogon的LTT均为阳性。Saiko的LTT在所有4例患者中均为阳性，Kanzo的LTT在4例患者中的3例中为阳性。在患者中未观察到嗜酸性粒细胞增多或肝损伤。在本研究中，我们对那些一生中没有服用汉方药物的患者进行了这些草药的LTT。这些结果表明，LTT的草药可能会产生假阳性结果和LTT是不可靠的诊断金宝药引起的肝损伤。