Relationship between membrane lipids and alcohol fermentation in Zymomonas mobilis

本文研究了运动发酵单胞菌的膜脂与酒精发酵的关系，发现运动发酵单胞菌中含有两种长链碱性类脂：一种是游离型神经酰胺，另一种是含葡萄糖醛酸神经酰胺。四羟基细菌烷(THBH)是一种新的生物体脂质，被认为是一种与高乙醇和高盐浓度的耐受性更相关的脂质。用薄层层析法从提取的脂类中分离得到一种含葡萄糖醛酸神经酰胺。脂类的水解得到三个化合物，一个长链碱，一个脂肪酸甲酯和一个糖甲酯。GC-MS分析表明，该糖酯为葡萄糖醛酸甲酯。长链碱和脂肪酸甲酯与游离神经酰胺相同。这三种化合物的摩尔比几乎相同。在不同添加乙醇的条件下，对菌体的脂肪组成进行了检测。THBH在乙醇浓度较高时增加，但其衍生物THBHester和THBH-糖苷减少。在不同KCl浓度的条件下，对其脂质成分进行了检测。THBH只有在高KCl浓度时才增加。

This study deals with relationship between membrane lipids and alcohol fermentation in Zymomonas mobilis, in which two long-chain base-containing lipids were found : one was a free ceramide and the other was a glucuronic acid-containing ceramide. Tetrahydroxybacteriohopane(THBH), a novel lipid of the organism, was thought to be a lipid more concerned with tolerance of high ethanol and a high salt concentrations.1. A glucuronic acid-containing ceramide was isolated from the extractable lipids by thin-layer chromatography. Hydrolysis of the lipid gave three compounds, a long-chain base, a fatty acid methyl ester and a sugar methyl ester. GC-MS analysis showed that the sugar methyl ester was that of glucuronic acid. The long-chain base and fatty acid methyl ester were the same those of the free ceramide. The three compounds were nearly same ratio in the molarity.2. The lipid composition of the organism was examined in the medium of various added ethanol. THBH increased in a high ethanol concentration, but the derivatives, THBHester and THBH-glycoside, decreased. The lipid composition was also examined in the medium of various KCl concentration. THBH only increased in a high KCl concentration.