Synthesis of Fungal potent auxins, accremoauxins, and their applied study on initiation and propagation of frail calli of conifers.

1)以玫瑰香I42671为原料，从（<正负>）- ipa中分离得到(+)-2-（3-吲哚基）丙酸[(+)- ipa]，并以甘甘醇为原料制备2,3,4,5 -二-0-异丙基-d -阿拉伯糖醇为原料，合成了新的强效生长素衍生物Acremoauxin A（AA-A）。合成证实的AA-A结构为1-0-[(2S)-2-（3-吲哚基）丙烯基- d -阿拉伯糖醇。其相关化合物AA-B由二异丙烯- d -阿拉伯糖醇和（3-吲哚基）乙酸（IAA）合成，在TLC、HPLC和^1H-NMR上与天然化合物一致。2)在Murasige & Skoog培养基上研究了含BA或不含BA的AA-A等多种生长素对杉木和赤松愈伤组织生长的影响。结果表明，愈伤组织萌发和繁殖的活性依次为：外植体：苏木三倍体b>赤松二倍体>，生长素：NAA， 2,4 - d> IBA, 5,6 - diaa >IPA, AA-A， IAA.3)研究了AA-A和IPA对映体对植物幼苗生长的影响。AA-A对白菜、生菜和燕麦幼苗的生长有明显的抑制作用，但对这些幼苗的抑制作用弱于IPA。在5种植物幼苗试验中，(+)-IPA的生长抑制作用高于其对映体。而(-)- ipa对水稻和萝卜幼苗生长的抑制作用强于(+)- ipa。

1)Acremoauxin A(AA-A), a new potent auxin derivative produced by Acremonium roseum I4267, was synthesized from 2, 3 : 4, 5-di-0-isopropylidene-D-arabitol prepared from Dmannitol and(+)-2-(3-indolyl)propionic acid[(+)-IPA, obtained from(<plus-minus>)-IPA by biological resolution using A. roseum I42671. The synthetically confirmed structure of AA-A was 1-0-[(2S)-2-(3-indolyl)propionyll-D-arabitol. Its related compound, AA-B chromatographically detected from this fungus, was similarly synthesized from the diisopropylidene-D-arabitol and(3-indolyl)acetic acid(IAA), and synthetic AA-B was in fair agreement with natural one in TLC, HPLC and ^1H-NMR.2)Effects of the var4-ous auxins including AA-A with or without BA on the growth of the calli from Sugi and Akamatsu were investigated on the Murasige & Skoog's media. Results indicated the following orders in activities of callus initiation and propagation. Explant : Sugi triploid>its diploid>Akamatsu, auxins : NAA, 2, 4-D>IBA, 5, 6-DIAA>IPA, AA-A, IAA.3)Effects of AA-A and the enantiomers of IPA on the growth of plant seedlings were examined. AA-A markedly inhibited the growth of the seedlings of Chinese cabbage, lettuce and oat, but its inhibitory activity against these seedlings was weaker than that of IPA. In tests with 5 species of plant seedlings, (+)-IPA showed the growth inhibition higher than that of its enantiomer. Two seedlings of rice and radish, however, were highly inhibited in the growth by(-)-IPA rather than(+)-IPA.