Studies on the color improvement of sugi wood by use of natural pigments

(1)糖心材的颜色分类测定了500份糖心材的L^*、a^*、b^*值，并根据L^*值的不同分为6组。测定了6组心材甲醇提取物的可见光光谱，阐明了黑素(黑素)和黑素(深褐色心材)的光谱性质。(2)对心材深褐色的脱色。在各种化学处理中，亚氯酸钠对心材的脱色效果最好，亚氯酸钠处理适合于心材的调色，因为脱色效果随着亚氯酸钠浓度的增加而线性增加。(3)利用甜菜胚芽愈伤组织培养产生红色色素。并在琼脂培养基中进行培养。对甜瓜愈伤组织产生微红色色素的培养条件进行了研究。愈伤组织的颜色与外植体(杉木)的心材颜色之间没有关系。在无氧琼脂等恶劣条件下生长缓慢的愈伤组织中，常能观察到产生红色素的愈伤组织，但对产生红色素的愈伤组织的培养条件尚不明确。通过上述实验，对植物激素与愈伤组织发育的关系以及愈伤组织中β-谷甾醇的产生进行了一些新的观察。

(1)Classification of sugi heartwood by colorThe values of L^*, a^* and b^* in 500 samples of sugi heartwoods were measured, and were classified into 6 groups by the difference of L^* values. VIS spectra of the methanol extracts from the 6 groups of sugi woods were measured, and spectroscopic properties of "akasin"(readish sugi heartwood)and "kurosin"(dark brown sugi heartwood)were elucidated.(2)Decolorization of the dark brown heartwoof of sugiIt was found that sodiuia chlorite decolorized most effectively sugi heartwoods among various chemical treatments, and the treatment of sodium chlorite was suited for the color conditioning of sugi heartwood since the effect of decolorization increased lineally with the concentration of sodium chlorite.(3)Production of reddish pigments by the callus culture deriving from the carabium of sugi woodThe techniques were established that a callus was derived from the cambium of sugi wood, and was cultured in an agar medium. Various experiments were conducted in order to find the culture condition producing the reddish pigments in the sugi callus. There was no relationship between the color of the callus and that of the heartwood in the explant(sugi wood). The callus having reddish pigments was often observed in the cali growing slowly under the severe condition such as in the inoistureless agar medium, but the culture condition of callus producing reddish pigment were not determined precisely. Through the above experiments, several new observations were obtained on the relationship between phytohormones and callus development, and on the production of beta-sitosterol in the sugi callus.