Immunohistochemical study on atrial natriuretic peptide in the ventricle of hearts of infants and children.

在本研究中，研究了人心房利钠肽（ANP）在婴幼儿心室的存在和分布。anp免疫、反应性肌细胞清晰、清晰、清晰。(1)心内膜纤维、血管狭窄、心包扩张等。distri。btit。1 . anp阳性细胞在左心室内三分之一最常见。在缺血性心脏病和非心脏病患者的任何心脏中均未检测到ANP免疫反应性。anp阳性心肌细胞的左心室容积大于anp阴性心肌细胞的左心室容积。anp阳性的niocyte的直径比anp阴性的心肌细胞大。对心内膜纤维弹性增生的心肌组织进行了显微组织化学检查。活检标本中anp阳性肌细胞的频率与左室舒张末期容积呈显著相关（r=0.735），与射血分数呈负相关（r=0.730）。与组织病理严重程度指数（心肌细胞肥大、变性、间质纤维化）相关（r=0.762）。超微结构检查证实心室肌细胞内存在特异的电子致密颗粒，直径250 ~ 400nm。免疫电镜显示在特定颗粒中存在anp标记胶体金颗粒。脑利钠肽在某些颗粒中与ANP共定位。本研究结果提示，婴儿期和儿童期心肌疾病患者心室中ANP的表达与心肌壁压力和心肌细胞压力过大有关。

In this study, the presence and distribution of human atrial natriuretic peptide (ANP) were i. iivestigated iijimtinoliistoclieniically in tlie ventricle of the hearts of infantsaiid children. ANP-immunoi, eactive myocytes were cleai, ly deinoiisti, at. e(i iii tlie veiiti, icle of liearts wi L. Ii endocardial fibi, oel-astosis and dilated cardioinyopat, iiy. The distri. btit. i. oii of ANP-positive inyocytes was most frequent iii the inner otie-third of tlie left ventricle. No ANP'immunoreactivity was detected in any lieart in cases with congei-iital heart disease or noncardiac disease. The left ventricular volume of liearts with ANP-positive myocytes was larger than that witli ANP-negetive myocytes. The inean diameter of ANP-positive niyocytes was greater tilail that of ANP-negative myocytes. Iminunohistochemical study was also carried out in the biopsied ventricular myocardium of endocardial fibroelastosis. The frequency of ANP-positive myocytes in the biopsied specimens significantly correlated with left ventricular end-diastolic volume (r=0.735) and inversely correlated with ejection fraction (r=0.730). It was also correlated with histopathologic severity index (inyocyte hypertrophy, degeneration and interstitial fibrosis) (r=0.762). Ultrastructural examination confirmed the presence of specific electrondense granules of 250-400 nm in diameter in the ventricular myocytes. Immunoelectron microscopy demonstrated the presence of ANP-labeling colloidal gold particles in the specific granules. It was also demonstrated that brain natriuretic peptide was colocalized with ANP in some of the granules. The results of this study suggest that ANP expression in the ventricle of heart muscle disease in infancy and childhood is related to excess of wall stresses and of myocyte stresses.