Image analysis of organ disorder caused by alcohol

1.方法取中、晚期尸检的肝脏和全脑组织碎片，用10%主液固定。在进行Azan和油红0染色后，拍摄肝脏的显微照片。在胼胝体、乳头体和顶枕沟的膝水平上拍摄大脑额叶断面照片。采用图像分析系统测定肝纤维化或脂肪化程度，并测量脑灰质和白色物质面积.结果纤维成分占总面积的0.72-3.91% 5例正常对照组平均2.55，1.68-4.63%，（3.36）脂肪肝7例，6.44-30.06% 9例脂肪肝合并肝硬化患者中，肝细胞癌的发生率为13.89%，占7.34-28.76%脂肪肝5例，脂肪成分占总面积的9.29-49.62%（24.89），正常对照组15例，额叶灰质占总面积的平均值为0.552。顶叶为0.501，颞叶为0.641，枕叶为0.735。额叶白色物质/总面积平均为0.448。顶叶、颞叶和枕叶的平均值分别为0.499、0.350和0.246。6例酗酒者额叶灰质/总面积的平均值为0.552。顶叶为0.535，颞叶为0.690，枕叶为0.672。额叶白色物质/总面积平均为0.478。顶叶0.465，颞叶0.672，枕叶0.328。酗酒者额叶、枕叶灰质萎缩，顶叶、颞叶白色物质萎缩。

1. MethodsFragments of liver and total brain which were taken out in the medio-legal autopsy were fixed with 10% formain solution. After Azan and oil-red 0 stains were carried out, microscopic photographs of liver were taken. Photographs of cerebral frontal sections at the level of Genu of corpus callosum, Mammillaly bodies and Parieto-occipital sulci were done. Using with image analysing system, degree of liver fibrosis or adiposis were determined and areas of cerebral gray seatter and white matter were measured.2. ResultsFiber component/total area was 0.72-3.91%(average 2.55)in the 5 normal cases, 1.68-4.63%(3.36)in the 7 cases of fatty liver, 6.44-30.06%(13.89)in the 9 cases of fatty liver with liver cirrhosis and 7.34-28.76%(18.11)in the 6 cases of liver cirrhosis.Fat componemt/total area were 9.29-49.62%(24.89)in the 5 cases of fatty liver.In the 15 normal cases, average of cerebral gray matter/total area was 0.552 in the frontal lobe. The one in the parietal lobe was 0.501, in the temporal lobe 0.641 and in the occipital lobe 0.735. Average of white matter/total area was 0.448 in the frontal lobe. The one in the parietal lobe was 0.499, in the temporal lobe 0.350 and in the occipital lobe 0.246.In the 6 brains of alcoholics, average of gray matter/total area was 0.552 in the frontal lobe. The one in the parietal lobe was 0.535, in the temporal lobe 0.690 and in the occipital lobe 0.672. Average of white matter/total area was 0.478 in the frontal lobe. The one in the parietal lobe was 0.465, in the temporal lobe 0.672 and in the occipital lobe 0.328.In alcoholics atrophy of gray matter in the frontal and occipital lobe were observed and the one of white matter were observed in the parietal and temporal lobe.