The role of eosinophil in allergic diseases.

因为对嗜酸性粒细胞的吞噬功能知之甚少。我检测了一些嗜酸性粒细胞的吞噬功能。与中性粒细胞相比，嗜酸性粒细胞的趋化反应水平降低。外周嗜酸性粒细胞的杀菌活性低于中性粒细胞。为了了解细胞质氧自由基在过敏反应中的作用，我检测了从有或没有过敏发作的患者以及非过敏对照获得的纯化的嗜酸性粒细胞和中性粒细胞。检测方法包括鲁米诺依赖化学发光法、超氧阴离子生成法和过氧化氢生成法。佛波醇12-肉豆蔻酸酯13-醋酸酯刺激后，三种参数的嗜酸性粒细胞产生的氧自由基均高于正常对照的中性粒细胞。处于稳定状态的过敏性受试者的嗜酸性粒细胞与正常对照组无明显区别。然而，在哮喘发作期间从患者身上获得的嗜酸性粒细胞比在两次发作之间从患者那里获得的嗜酸性粒细胞反应更快。患者在发作期间或发作之间的中性粒细胞表现出相同的特征。结果提示，嗜酸性粒细胞释放的氧自由基可能在哮喘发作过程中对组织的损伤起着重要作用。

Since little is know about the phagocytic functions of eosinophils. I have examined some phagoeytic functions-of eosinophils.Eosinophils-showed decreased levels of chemotactic response, as compared with neutrophils. / The peripheral eosinophils had a lower bactericidal activity than neutrophils.In order to understand the role of cytoplasmic oxygen radicals in allergic reaction, I tested purified eosinophils and neutrophils obtained from patients with or without allergic attacks as well as from non-allergic controls. The test methods included luminol dependent chemiluminescence, superoxide anion production and hydrogen peroxide production. Stimulation with phorbol 12-myristate 13-acetate induced higher production of the oxygen radicals in eosinophils than-in, -neutrophils of normal controls in all three parameteres. Eosinophils of allergic subjects in stable conditions behaved indistinguishable from that of normal controls. however, eosinophils obtained from patients during attacks of bronchial asthma were more responsive than those from patients between attacks. Neutrophils of patients during or between attacks showed the same profiles. The results suggested that the oxygen radicals released from eosinophils may have a significant role to injure the tissue during the bronchial asthma attack.