Basic Research on Hormonal Treatment of Pancreatic Cancer

1.人胰腺组织雌二醇(E_2)和雌激素受体(ER)的研究免疫组织化学检测22例中有17例出现E2阳性细胞。急诊室几乎是阴性的。结论：1.冰冻组织中ER生化检测为阴性。胰腺癌组织中表皮生长因子、表皮生长因子受体和c-erbB-2蛋白的免疫组织化学表达胰腺癌组织中EGF、EGFR和c-erbB-2蛋白阳性率分别为72%、36%和28%。这三种蛋白检测呈阳性的患者的生存期在统计学上比检测为阴性的患者要短。用核仁组成区(NORs)银染法研究人胰腺癌细胞的增殖能力。胰腺良性(正常：1.86；+-gt；0.38，胰腺炎：2.14；+-gt；0.53)和恶性(4.02；+-gt；1.19)胰腺组织(p&lt；0.01)之间的Ag-NORs指数有显著差异。胰腺癌的AG-NORs指数明显低于胃癌组(6.35；+-1.32)和结肠癌(7.66；+-MI4)。以Wistar-Bonn/Kobori大鼠(WBN/KOB)为模型，研究了WBN/KOB自发性胰腺炎和糖尿病的致癌作用。氮杂丝氨酸(50 mg/只，ip)不能诱发大鼠胰腺癌。接种7，12-二甲基苯并(A)菲诱发大鼠胰腺导管细胞样癌。

1. Studies on estradiol (E2) and estrogen receptor (ER) in human pancreatic tissues. E2-positive cells were immunohistochemically found in 17 of 22 cases. ER was almost negative. Biochemical assay of ER in frozen tissue was negative.2. Immunohistochemical expressions of epidermal growth factor (EGF), epidermal growth factor receptors and c-erbB-2 oncoprotein in human pancreatic cancer. The positive rates of EGF, EGFR and the c-erbB-2 oncoprotein in pancreatic cncer tissues were 72%, 36% and 28%, respectively. The survival period for patients who tested positive for these three proteins was statisticaly shorter than for those who tested negative.3. The proliferating potential of human pancreatic cancer cells were studied by Ag-Nucleolar Organizer Regions (NORS) staining. A significant difference in Ag-NORs index was found between benign (normal : 1.86<plus-minus>0.38, pancreatitis : 2.14<plus-minus>0.53) and malignant (4.02<plus-minus>1.19) tissues of pancreas(p<0.01). Ag-NORs index in pancreatic carcinoma showed significantly lower value than that in cancer of stomach (6.35<plus-minus>1.32) and colon (7.66<plus-mi4. The Wistar Bonn/ Kobori rat (WBN/Kob) that is induced pancreatitis and diabetes mellitus spontaneously was studied for the carcinogenesis. Azaserine (50 mg/body, ip) did not induce pancreatic cancer in this rat. Inoculation of 7, 12-dimethylbenz(a)anthracene induced the duct cell-like carcinoma in pancreas of this rat as well as conventional Wistar rat.