Continuous measurement of lactate concentration in cerebral interstitium as an index of regional brain glucose metabolism in rats.

[1]使用微透析进行连续乳酸盐测量。Shock，1992，通过透析液与试剂溶液的在线混合连续测量labtate浓度。响应时间约为4分钟，这对于测量由脑活动变化引起的葡萄糖代谢的变化来说不够快。因此，我们应用该在线系统测量急性出血后不久大鼠骨骼肌间质乳酸浓度。从血液中的乳酸浓度和肌肉中的乳酸浓度的比较，这表明，大鼠骨骼肌功能作为一个乳酸消费者，而不是乳酸生产者急性出血后。这一假设得到了证实，通过使用在美国的体内校准方法。J. Physiol.1992. [2]用微透析法间歇测定不同模型脑间质乳酸含量在缺氧、缺氧和内毒素休克模型中，每隔10 ~ 30 min测定一次脑间质乳酸含量。这些研究表明，脑乳酸浓度保持在相对较低的水平，而血乳酸显着增加。（已提交出版）。还发现，GABA相关的药物，包括巴比妥类药物增加局部脑乳酸浓度后，与这些物质添加在微透析灌注液中的局部刺激。[3]用微透析法测定内源性神经活性物质在清醒大鼠中测定出血诱导的局部脑促甲状腺素释放激素（TRH）释放。出血后，隔区TRH浓度显著升高，但在其他脑区仍保持在基础水平（Life Science 1991）。

[1]Continuous lactate measurement using a microdialysisAs shown in Circ. Shock, 1992, labtate concentration was measured continuously via the on-line mixing of dialysate with a reagent solution. The response time was approximately 4 min, which was not rapid enough for measurement of changes in glucose metabolism caused by brain activity changes. Therefore we applied this on-line system to measurement of the interstitial lactate concentrations in skeletal muscle interstitium of the rat shortly after an acute hemorrhage. From the comparison of lactate concentrations in blood and in the muscle interstitium, it was suggested that rat skeletal muscle functions as a lactate consumer, rather than a lactate producer after acute hemorrhage. This hypothesis was confirmed by using in vivo calibration method in Am. J. Physiol. 1992.[2]Intermittent measurement of cerebral interstitial lactate in various models by a microdialysisCerebral interstitial lactate was measured with 10 - 30 min intervals in hypoxic hypoxia and in endotoxin shock models. These studies revealed that cerebral lactate concentration remained relatively at low levels while blood lactate increased markedly. (Submitted for publication). It is also found that GABA-related drugs including barbiturates increased local cerebral lactate concentration after local stimulation with these substances added in the microdialysis perfusate.[3]Measurement of endogenous neuroactive substances by a microdialysisHemorrhage-induced regional brain thyrotropin-releasing hormone(TRH)release was measured in conscious rats. The TRH concentration significantly increased in the septum after a hemorrhage but remained at basal levels in the other brain areas measured(Life Science 1991).