Screening of sperm motility stimulating substances, and their application to male infertility

1本研究建立的玻璃柱-连续密度梯度离心法能够从人的生精液中逐步分离出活动精子。该制剂不含精浆成分，适合于用计算机辅助精子运动分析仪定量测定精子活力。2.用计算机辅助精子运动分析仪评价生物活性多肽、缓激肽、血管紧张素Ⅱ和脑啡肽对高纯度人精子活力的影响。它们刺激了精子的活力，特别是在没有精浆的情况下提高了精子的前进速度。然而，加入精浆后，这种作用消失了。精浆中的蛋白水解酶可能使其失活。3在精浆存在的情况下，磷酸二酯酶抑制剂戊氧西林(PXF)可刺激精子活力。在逆行射精的情况下，精液在膀胱中回流，甚至替换尿液到培养液中，尿液污染降低了精子存活率。射精前膀胱中引入PXF的培养液对精子存活率有保护作用。回收的精子无活力丧失，用于IVF-ET，成功妊娠1例。

1 The glass column-continuous density gradient centrifugation, established in the present study, was capable of separating progressively motile sperm from raw human semen. The preparation was free from seminal plasma components, and was suitable for quantitative measurement of sperm motility by using the computer assisted sperm motility analyzer.2 Effect bioligically active peptides, bradykinin, angiotensin II and enkephalin, on highly purified human sperm motility were estimated by using the computer assisted sperm motility analyzer. They stimulated sperm motility, especially for sperm-progressive velocity in the absence of seminal plasma. The effects were, however, disappeared by addition bf seminal plasma. Proteinases in seminal plasma might inactive them.3 Pentoxifilline(PXF), a phosphodiesterase inhibitor, stimulate sperm motility in the presence of seminal plasma. In the case of retro-grade ejaculation, semen was refluxed in blader, and even replaced urine to culture medium, contamination of urine decreased sperm viability. The culture medium with PXF intorduced in blader before ejaculation protected sperm viability. The recovered sperm without loss of motility was provided for IVF-ET, resulting one successful pregnancy.