Multipatch Setup

我们在此申请采购多贴片装置，使美因茨大学医学中心的神经科学研究小组能够进行复杂的电生理实验。多面片设置可以直接分析网络参数，如连通性、兴奋和抑制，对单个动作电位的时间精确检测，以及突触特性(短期可塑性、突触强度等)的分析。在急性脑片中。原则上，对突触交流的分析也可以在记录形式2神经元的传统设置上进行。然而，这些测量非常耗时，因为在急性脑片中发现单通道连接对的概率非常低。使用建议的多面体设置，可以同时记录多达12个神经元。此外，装置上的移液管可以在每次记录后自动清洁，这样就可以用一个移液管连续记录来自多个神经元的信号。这极大地增加了一个实验(&gt；100)可以测试的神经元连接的数量。在多贴片设置的帮助下，可以在短时间内获得大量的电生理数据，而大大减少了实验动物的数量。这将使我们能够对急性脑片中的复杂网络参数进行全面分析，这在传统设置下是不可能的，因为需要花费很高的时间。

We hereby apply for the procurement of a multipatch setup that will enable neuroscience researching groups at the University Medical Center Mainz to carry out complex electro-physiological experiments. A multipatch setup enables a direct analysis of network parameters such as connectivity, excitation and inhibition, time-precise detection of individual action potentials, and the analysis of synapse properties (short-term plasticity, synaptic strength, etc.) in acute brain slices. In principle, the analysis of synaptic communication is also possible on conventional setups with recording form 2 neurons. However, these measurements are very time-consuming, since the probability of finding monosynaptically connected pairs in acute brain slices is extremely low. With the proposed multipatch setup, up to 12 neurons can be recorded simultaneously. In addition, the pipettes on the setup can be cleaned automatically after each recording such that one can record with one pipette signals from several neurons in succession. This drastically increases the number of neuron connections that can be tested in one experiment (>100). With the help of a multipatch setup, large amounts of electrophysiological data can be obtained in a short time with a significantly reduced number of test animals. This will enable us to carry out a comprehensive analysis of complex network parameters in acute brain slices, which would not be possible with conventional setups the high expenditure of time.