Co-operative Responses of Organella Constituents to Drug stimulation

考虑到推进本项目研究的效率，本课题组根据细胞器组成结构域可分为细胞膜、细胞质和细胞核的思想，将其分为三个亚组：(1)细胞膜功能分子的相互作用和第二信使与胞液因子的表达：磷脂(Akamatsu)和Chlesterol(Inoue)的生物合成及其向细胞膜的转运。(2)细胞质中功能分子的活性行为：抗锥虫药物对过氧化体形成的影响(Takano)，G蛋白与肥大细胞的相互作用(Ichikawa)和5-羟色胺受体(Nomura)，多胺对核糖体功能的影响(Igarashi)，环ADP核糖的贡献(Okamoto)。(3)外源性刺激剂对核功能分子的影响及其反应：甲状腺反应的基因表达调控(Ohba)，类固醇(Noda)和胰升糖素(Ichiyama)激素刺激G蛋白对核膜形成的贡献(Natori)13EA03：在研究会上，我们根据各自的结果讨论了外源信息如何通过转导机制转化为第二信使，最终到达目的基因。我们的结论是，从细胞表面受体到靶基因的信息传递应该有很多途径，其中Okamoao发现的环状ADP核糖的贡献肯定是非常重要的。进一步的研究应该集中在信息传递的稳态通道如何被外来信息，即新给药或抑制剂干扰的问题上。这些观察结果可能会为药物作用的分析提供许多建议。

On considering the efficiency for promoting our project research, this research group was divided into three subgroups depending on the idea that organella consisting domain can be classified to cell membrance, cytoplasm and cell nucleus.(1)The interaction of functional molecules in cell membrane and expression of 2nd messenger with cytosolic factors : the biosynthesis of phospholipid (Akamatsu) and chlesterol (Inoue) and their transport to cell membrance. (2)The active bahavior of functional molecules in cytoplasmic organella : effect of anti-trypanozoma drug to peroxisome formation (Takano), interaction of G protein with mast cells (Ichikawa) and serotonin receptor (Nomura), effects of polyamines to ribosomal function (Igarashi), and contribution of cyclic ADP ribose (Okamoto).(3)The effect of exogenous stimulant to nuclear functional molecules and the response : regulation of gene expression for the response to thyroid (Ohba), steriod (Noda) and glucagon (Ichiyama) hormone stimulation contribution of G protein to the formation of nuclear membrane (Natori)..13EA03 : In the research meeting we discussed on basis of each own result about the processes how exogenous information is converted to 2nd messenger through transduction mechanism and finally attain to the target gene. We concluded that there should be enormous ways of information transfer from cell surface receptor to the target gene, among which contribution of cyclic ADP ribose, discovered by Okamotao must be very important. The further research should be conncentrated to the point that how the steady state passway of information transfer can be disturbed by the foreign information, that is newly administered drugs or inhibitors. These observations may offer many suggestions to the analysis of drug action.