Novel peptidylglycans with hemagglufinating activity-chemical structure and biological activity

我们发现，11种海洋藻类具有新的血凝素，不能用磷酸盐缓冲液提取，除非藻类已经用链霉蛋白酶预处理。新的血凝素已被分离出的链霉蛋白酶处理的提取物的红藻，石花菜和扇叶果，分别。“链霉蛋白酶处理依赖性”血凝素通常是表观分子量分别为8 x 10^5的肽基聚糖。糖链中主要单糖为半乳糖和木糖，糖醛酸、3，6-脱水半乳糖和硫酸酯也是主要成分。肽段具有相似的氨基酸组成，富含Ser、Gly和Glx，表明它们的分子中分别存在Xyl-Ser键。“链霉蛋白酶处理依赖性”血凝素显著地凝集绵羊红细胞，尤其是胰蛋白酶或链霉蛋白酶处理的红细胞，并且还显示对小鼠脾淋巴细胞的促有丝分裂活性。他们是稳定的，在高温下，在较宽的pH值范围内，而增加在低pH值。的活性弱抑制去唾液酸胎球蛋白和NaCl，但不是由其他糖化合物测试。活性不受高碘酸氧化或在用4 M胍-HCl的解离状态下以及用酶如蛋白酶、糖苷酶和硫酸酯酶消化的影响，这表明它们具有耐化学或酶攻击的化学结构。

We found that eleven species of marine algae possessed novel hemagglutinins, which could not be extracted with phosphate buffered saline, unless the algae had been pretreated with Pronase. The novel hemagglutinins have been isolated from the Pronase-treated extracts of the red algae, Gelidium amansii and Carpopeltis flabellata, respectively. The 'Pronase treatment-dependent' hemagglutinins were commonly peptidylglycans with the apparent molecular weight of 8 x 10^5, respectively. As to glycan moieties, the major monosaccharides were Gal and Xyl, and uronic acids, 3,6-anhydro-galactose and sulfate esters also detected as the constituents. The peptide moieties share similar amino acid composition rich in Ser, Gly and Glx, suggesting that Xyl-Ser linkages in their molecules, respectively.The 'Pronase treatment-dependent' hemagglutinins markedly agglutinated sheep erythrocytes, especially trypsin- or Pronase treated ones, and also showed mitogenic activity for splenic lymphocytes of mice. They were stable at a high temperature and in a wide range of pH with rather increase at a low pH. The activity was weakly inhibited by asialofetuin and NaCl,but not by other sugar compounds tested. The activity was not affected by periodic acid oxidation or under the dissociation state with 4M guanidine-HCl as well as digestion with enzymes such as protease, glycosidases and sulfatase, which suggest that they have chemical structures resistant to chemical or enzymatic attacks examined.