DIAGNOSIS OF INFLAMMATORY DISEASES BY QUANTITATIVE MEASUREMENT OF SERUM ACUTE-PHASE REACTIVE PROTEINS IN HORSES.

急性期反应是针对感染、组织损伤、肿瘤生长或免疫性疾病的早期生物过敏反应。一些血浆蛋白作为急性时相蛋白，在炎症刺激后的急性时相反应中发生特征性变化。（α_2 MG），α_1-酸性糖蛋白分离、纯化并鉴定了血清α_1AG、铜蓝蛋白（CP）、C-反应蛋白（CRP）、触珠蛋白（HG）、血清酸溶性蛋白（ASP）和血清淀粉样蛋白A（SAA），并建立了马血清中这两种蛋白的客观测定方法。然后测定了正常增龄马和围产期马血清中上述三种物质的浓度。在实验诱导炎症的马中，SAA显示出对炎症刺激的最早反应，并且其浓度在治疗后第2天增加到治疗前值的几十倍。alpha_1AG是 ...更多信息 第二个增加，在第2 - 3天是治疗前值的1.5 - 2倍，然后是CRP，在第3 - 4天增加5 - 6倍。第4 ~ 5天HG升高1.5 ~ 9倍，第5天ASP升高1.8 ~ 1.9倍。CP反应缓慢，第6 ~ 7天浓度增加1.5 ~ 2倍。马α_2MG不是急性时相反应蛋白。尽管在本研究中收集了具有炎症临床体征的马的血清，但未注意疾病的临床阶段或状况，但与正常值相比，大多数临床病例中的SAA浓度在统计学上较高。此外，这些临床病例显示，与其他急性时相蛋白、α_1AG、CRP、HG、ASP和CP相比，SAA浓度较高。在这一点上，没有解释的差异，在响应时间为每种蛋白质，所以详细调查的功能，每种蛋白质是necessary.In目前的结果，SAA的反应是最敏感的，并增加到一个更大的水平比其他急性反应蛋白。因此，马SAA被认为是最敏感的急性时相反应蛋白，因为在急性炎症中观察到的水平增加。少

The acute-phase response is a biophylactic reaction at an early stage against infection, tissue injury, neoplastic growth, or immunological disorders. Some plasma proteins as acute-phase proteins change characteristically with the acute-phase response following inflammatory stimuli.In this study, equine acute-phase reactive proteins, alpha_2-macroglobulin (alpha_2MG), alpha_1-acid glycoprotein(alpha_1AG), ceruloplasmin(CP), C-reactive protein (CRP), haptoglobin(HG), serum acid soluble protein(ASP) and serum amyoid A protein(SAA) were isolated, purified and characterized, and objective measurments of them in equine serum were established. Then, serum concentrations of them were determined in normal horses with aging and in mares during perinatal period. In horses with experimentally induced inflammation, SAA showed the earliest reaction against inflammatory stimuli, and its concentration increased to several ten times the pre-treatment value on the 2nd day after treatment. alpha_1AG was … More the next to increase, 1.5 to 2 times the pre-treatment values on the 2nd to 3rd day, followed by CRP which increased 5 to 6 times on the 3rd to 4th day. HG was elevated 1.5 to 9 times on the 4th to 5th day, and ASP increased to 1.8 to 1.9 times on the 5th day. The response of CP was slow, its concentration increasing 1.5 to 2 times on the 6th to 7th day. Equine alpha_2MG was not an acute-phase reactive protein. Though sera from horses with clinical signs of inflammation were collected without noting the clinical stage or conditions of the disease in this study, SAA concentrations in most clinical cases tested were statistically high compared with the normal value. In addition, these clinical cases showed high SAA concentration to be dominant compared to other acute-phase proteins, alpha_1AG, CRP, HG, ASP and CP. At this point there is no explanation for the differences in response time for each protein, so detailed investigation of the functions of each protein is necessary.In the present results, the response of SAA was the most sensitive and increased to a greater level than other acute-reactive proteins. Equine SAA was therefore concluded to be the most sensitive acute-phase reactive protein because of the increased levels seen with acute inflammation. Less