Production of Interleukin-6 on Periodontal cells

白细胞介素6(IL-6)是一种多功能细胞因子，以前被称为B细胞刺激因子2、肝细胞刺激因子、干扰素-β2，由淋巴系和非淋巴系细胞产生。该细胞因子可通过作用于破骨细胞而明显诱导破骨细胞性骨吸收。此外，有报道称，牙周炎患者的牙周组织中含有较高水平的IL-6。因此，我们检测了人牙龈成纤维细胞(Gin细胞)和人牙周膜成纤维细胞(PDL细胞)是否具有合成和分泌IL-6的能力，以响应直肠弯曲杆菌(C.rectus)和牙髓卟啉单胞菌(P.endodontalis)的内毒素。用抗IL-6的单抗，用ELISA法测定经内毒素处理后的细胞条件培养液中IL-6的含量。用Northern印迹杂交检测IL-6的表达。IL-6的释放和…的表达直肠弯曲菌内毒素刺激Gin细胞产生更多IL-6mRNA。另一方面，纤溶酶原激活物-纤溶酶系统参与了细胞外基质的降解，通过激活潜伏的胶原酶和前激肽释放酶参与炎症反应。在本研究中，我们检测了直肠直肌内毒素对人牙周成纤维细胞(Gin-1)纤溶酶和纤溶酶原激活物活性的影响。直肠弯曲菌内毒素刺激Gin-1条件培养液中纤溶酶活性的作用呈时间和剂量依赖性。直肠弧菌内毒素刺激Gin-1条件培养液和细胞裂解液中的纤溶酶原激活物活性。酶切鉴定Gin-1纤溶酶原激活剂的分子大小约为50 kDa。此外，经直肠弯曲菌内毒素处理的Gin-1条件培养液可促进血清中前激肽释放酶向激肽释放酶的转化，提示直肠弯曲杆菌和牙髓杆菌内毒素可能通过刺激IL-6或纤溶酶原激活物-纤溶酶系统的释放，在牙周炎的炎症和细胞外基质的降解中起重要作用。较少

Intherleukin-6 (IL-6), a multifunctional cytokine that has previously been called B-cell stimulatory factor 2, hepatocyte stimulating factor, interferon- beta _2,is produced by lymphoid and non-lymphoid cells. This cytokine can apparently induce osteoclastic bone resorption through an effect on osteoclastogesis. Furthermore, it has been reported that human gingival tissue from patients with periodontitis contain a high level of IL-6. Thus, we have examined whether human gingival fibroblast cells (Gin cells) and human periodontal ligament fibroblast cells (PDL cells) have the capacity to synthesize and secrete IL-6 in response to Campyrobacter rectus (C. rectus) LPS and Porphyromonas endodontalis (P. endodontalis) LPS. IL-6 in conditioned medium from these cells by treatment of LPS was measured ELISA assay using monoclonal antibody against IL-6. The expression rate of IL-6 mRNA was also investigated by Northern blot DNA hybridization with IL-6 cDNA probe.IL-6 release and the expression … More of IL-6 mRNA on Gin cells were stimulated by C. rectus LPS. And IL-6 release and the expression of IL-6 mRNA on PDL cells were stimulated by P. endodntalis LPS.On the other hand, plasminogen activator-plasmin system is implicated the degradation of extracrllular matrix, the inflammation through activating latent collagenase and prekallikrein. In this study we have measured th activities of plasmin and plasminogen activator on human gingival fibroblast cells (Gin-1) treated by of C. rectus LPS. Plasmin activity in the conditioned medium of Gin-1 was stimulated by treatment of C. rectus LPS in a time and dose-dependent manner. C. rectus LPS stimulated plasminogen activator activity in Gin-1 condition medium and cell lysate. The molecular size of plasminogen activator of Gin-1 was identified about 50 kDa by zymogrphy. Furthermore, Gin-1 conditioned medium treated with C. rectus LPS stimulated the conversion of prekallikrein to Kallikrein in serum.These data suggested that C. rectus LPS and P. endodontalis LPS is a potent stimulator in the inflammation and the degradation on the extracellular matrix of periodontitis through stimulates the releases of IL-6 or plasminogen activator-plasmin system. Less