Study on the Applicability of Common Mermosets in Developmental Toxicity Tests

为建立普通绒猴（Callithrix jacchus）发育毒性试验方法，收集本底资料，本研究选用20对在我所出生的普通绒猴。通过短期交配（分娩后7 - 14天）获得妊娠动物。妊娠率为43%，流产率为8%。通过从腹壁触诊子宫来诊断妊娠，从第45天开始可以诊断妊娠。于妊娠第110天剖腹产110日龄胎儿平均体重10.2g，体长60 mm，尾长58 mm，主要脏器平均重量：心脏84 mg，肺249 mg，肝脏343毫克孕100天胎儿主要骨骼发育尚不成熟。在妊娠第120天观察到死胎，但所有其他胎儿均存活，未发现畸形。对普通绒猴胎儿的影响。在妊娠第50 - 52天给予30 mg和300 mg/kg/天（N = 2）维甲酸，在妊娠第50 - 56天给予50 mg（N = 2）或100 mg和300 mg/kg/天（N = 3）维甲酸。50 mg^- 7天组中有1只动物（50%），300 mg^- 7天组中有2例（66%），100 mg^- 7天组中有1只动物（10%）观察到胎仔死亡。此外，还观察到包括颞骨畸形（56%）、下颌骨发育不良（44%）、尾骨缺损（11%）和四肢骨畸形（11%）在内的骨骼畸形。

The purpose of this study was to establish a developmental toxicity test using common marmoset (Callithrix jacchus) and collect background data.Twenty pairs of common marmosets born in our institute were used. Pergnant animals were obtained by short-term mating (between 7 and 14 days after delivery). The gestation rate was 43% and the abortion rate was 8%. Pregnancy was diagnosed by palpation of the uterus from the abdominal wall and pregnancy could be diagnosed from the 45th day. Caesarian section was performed on the 110th day of gestation (109-111th day of gestation) and the 100th or 120th day of gestation.The mean body weight of the fetuses on the 110th day of gestation was 10.2g, the body length 60 mm and the tail length 58 mm. The mean weights of the main organs were 84 mg for the heart, 249 mg for the lungs, and 343 mg for the liver. Growth of the main bones in fetuses on the 100th day of gestation was still comparatively immature. Dead fetuses were observed on the 120th day of gestation, but all of the other fetuses survived and no deformations were found.The effects of retinoic acid (All-trans retinoic acid, Sigma Chemical Co.) on the fetuses of common marmosets were studied. Retinoic acid was administered at doses of 30 mg and 300 mg/kg/day (N = 2) on days 50 - 52 of gestation, and 50 mg (N = 2) or 100 mg and 300 mg/kg/day (N = 3) on days 50 - 56 of gestation.Abortions occurred in all marmosets in the 30 mg^- 3 day group, one animal- (50%) in the 50 mg^- 7 day group and two cases (66%) in the 300 mg^- 7 day group, Fetal death was observed in one animal (10%) in the 100 mg^- 7 day group. Skeletal anomalies including temporal anomalies (56%), hypoplastic mandibles (44%), tail bone defects (11%) and limb bone anomalies (11%) were also observed.It was found that common marmosets showed the same malformations as other primates when administered retinoic acid.