Chemical Developmental Toxicity Test Using A New Material Introducing Technique for Medaka Egg

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We developed a novel method of chemical developmental toxicity assay using pulsed power electroporation chemical transfer technique for fertilized medaka egg. We could find the best pulsed power treatment condition which introduced chemicals efficiently and did not affected embryogenesis and gene expression. Then we measured uptake of bisphenol A under this condition by GC-MS. Bisphenol A concentration in egg extract was higher than immersed solution. According to DNA microarray data, we found the specific gene expression profile by uptake of bisphenol A.