Development of the Resins having Antibacterial Activity

用氯化氢处理大网状甲基丙烯酸缩水甘油酯-二乙烯基苯共聚物小球，制备了含氯羟基的共聚小球。用胺或膦处理RCG或苯乙烯四乙二醇二甲基丙烯酸酯(RCS4G)，制备了含季铵盐基或膦基团的共聚微珠。以三乙胺、N，N-二甲基-正辛胺、N，N-二甲基-正十二胺(DMDA)、N，N-二甲基正十六胺为胺。三乙基膦(TEP)、三丁基膦(TBP)、三辛基膦(TOP)等用作磷化氢。研究了季铵盐型树脂(RCG-Q或RCS4G-Q)在不同条件下对苯磺酸钠(SBS)、十二烷基苯磺酸钠(SDBS)和细菌的吸附性能。RCG-Q和RCS4G-Q对大肠杆菌和金黄色葡萄球菌均有抗菌活性。RCG-Q可以在较宽的pH范围(4.5-8.5)和离子强度(0-0.3)范围内吸附SBS和SDBS。RCG-TOP(OH型)在水中对大肠杆菌有抗菌活性，而RCG-TEP和RCG-TBP没有抗菌活性。扫描电子显微镜显示细菌吸附在这些树脂上。树脂的抗菌活性和吸附能力不仅与季铵盐基或膦基团的结构有关，而且还与引入这些官能团的共聚微珠的基质种类有关。

Copolymer beads having chlorohysroxyl group (RCG) were prepared by treating macroreticular glycidyl methacrylate-divinylbenzene copolymer beads with hydrogen chloride. Copolymer beads bearing quaternary ammonium groups or phosphonium groups were prepared by treating RCG or stirene tetraethyleneglycol dimethacrylate(RCS4G) with amines or phosphines. Triethyl amine, N, N-dimethyl-n octhylamine N, N-dimethyl-n-dodecylamine(DMDA), N, N-dimethy-n-hexadecylamine were used as amine. Triethyl phosphine (TEP), Tributyl phosphine (TBP), and Trioctyl phosphine (TOP), and so on were used as phosphine. The adsorption of sodium benzenesulfonate(SBS), sodium n-dodecylbenzenesulfonate (SDBS), and bacteria with the resins containing quaternary ammonium groups(RCG-Q or RCS4G-Q) was investigated under various conditions. And their antibacterial activity was examined against Escherichia col (E.coli) and Staphylococcus aureus (S.aureus).RCG-Q and RCS4G-Q had antibacterial activity against E.coli and S.aureus.SBS and SDBS could be adsorbed with RCG-Q from the solution over wide pH(4.5-8.5) range and with ionic strength (0-0.3). RCG-TOP(OH type) exhibited antibacterial activity against E.coli in water, but RCG-TEP and RCG-TBP had no activity. Scanning electron micrograph showed that bacteria were adsorbed on those resins. Both antibacterial activity and adsorption ability of the resins were not only affected by the structures of quaternary ammonium groups or phosphonium groups but also the kinds of matrix of copolymer bead into which those functional groups introduced.