THE DEVELOPMENT AND THE CLINICAL APPLICATION OF AN OPTICAL SYSTEM FOR THE SIMULTANEOUS DETERMINATION OF METABOLIC AND FUNCTIONAL CHANGES IN THE HEART AND BLOOD VESSELS.

(1)开发同时测定心脏和血管代谢和功能变化的系统：与日本东京的日本光谱公司合作，成功地设计和制造了一种具有同心光纤的前表面荧光仪，用于双波长激发（内圈石英纤维）和荧光检测（外圈玻璃纤维）（CAM of -2）。(2)采用载fura-2的主动脉瓣条前表面荧光法，首次定量记录内皮细胞原位胞浆钙浓度（Ca）i。内皮素(ET)-1和ET-3在内皮细胞原位升高（Ca）i。ET-1升高（Ca）i为峰值型（第一阶段）和持续型（第二阶段）。第二阶段完全是细胞外钙依赖，钙内流。百日咳毒素（IAP）明显抑制第二阶段。因此，内皮细胞中由ET-1诱导的钙内流是由一种对内酰胺敏感的g蛋白原位调控的。(3)观察了乙醇对带内皮和不带内皮、α -毒素渗透后的猪冠状动脉条的收缩力以及对主动脉瓣内皮细胞的原位影响。研究发现，乙醇通过gtp结合蛋白介导的增加（Ca）i和提高收缩器官的Ca敏感性来收缩冠状动脉。当内皮暴露于乙醇中时，这种收缩被阻止，可能是通过释放内皮源性松弛因子（EDRF）。(4)采用前表面荧光法和装载bcecf的离体灌注大鼠心脏，成功记录心肌缺血-再灌注时pH值的变化。发现血管紧张素转换酶抑制剂卡托利能部分抑制缺血时酸中毒的发生。

(1) The development of a system for the simultaneous determination of metabolic and functional changes in the heart and blood vessels : A front-surface fluorometer with concentric optical fibers for double-wave length excitation (inner circle quartz fibers) and fluorescence detection (outer circle glass fibers) (CAM OF-2) was successfully and specifically designed and made with the collaboration of Japan Spectroscopic Co., Tokyo, Japan.(2) Using front-surface fluorometry of fura-2-loaded aortic valvular strips, the cytosolic Ca concentration, (Ca)i, of the endothelial cells in situ was firstly and quantitatively recorded. Both endothelin (ET)-1 and ET-3 elevated (Ca)i in the endothelial cells in situ. ET-1 elevated (Ca)i of a peak (the first phase) and sustained (the second phase) type. The second phase was exclusively extracellular Ca-dependent, Ca influx. Pertussis toxin (IAP) markedly inhibited the second phase. Thus, Ca influx induced by ET-1 is regulated by an IAP-sensitive G-protein in the endothelial cells in situ.(3) The effects of ethanol on the contractility of strips of porcine coronary artery, with and without endothelium, and following permeabilization with alpha -toxin, and on aortic valvular endothelial cells in situ were examined. It was found that ethanol contracted the coronaty artery both by increasing (Ca)i and by raising Ca sensitivity of the contractile apparatus, as mediated by GTP-binding protein. When the endothelium was exposed to ethanol, this contraction was prevented, presumably by releasing endothelium-derived relaxing factor (EDRF).(4) Using front-surface fluorometry and BCECF-loaded isolated perfused rat heart, pH changes of the myocardium was successfully recorded during ischemia-reperfusion. It was found that captoril, an angiotensin converting enzyme inhibitor, could partially inhibit the development of acidosis during ischemia.