Study on the determination of algal class's contributions to total-chlorophyll concentration from characteristic carotenoids of algal classes.

用高效液相色谱法测定了藻类光合色素、叶绿素(Chl)a、Chl c和类胡萝卜素含量。通过优化溶剂体系，建立了高灵敏测定海洋样品中藻类色素的反相高效液相色谱分析方法。该系统可以处理大于400微升的进样量，而不会显著降低叶绿素a和叶绿素c_1+c_2之间的分辨率。该系统还将分析贫营养海水的典型色素所需的样本量减少到500毫升。通过多元回归分析，分析了硅藻、双藻、甲藻、隐藻和雷氏藻类等藻类的类胡萝卜素特征，得到了藻类对总叶绿素a的贡献。硅藻、藻藻和甲藻对叶绿素a的贡献与根据细胞计数和观察到的细胞大小计算的结果进行了比较；估计的藻类丰度与色素和细胞计数之间有很好的相关性。

Algal photosynthetic pigments, chlorophyll (Chl) a, Chl c and carotenoids were determined by high-performance liquid chromatography (HPLC). A reversed-phase HPLC method was developed by optimizing a solvent system for the highly sensitive determination of algal pigments in marine in situ samples. The proposed HPLC system can handle injection volumes grater than 400 mul without a significant loss in the resolution between chlorophyllide a and chlorophyll c_1+c_2. The system also reduces the sample volumes required for the analysis of typical pigments of oligotrophic sea water to 500 ml. Carotenoids characteristic to algal classes such as the Bacillariophyceae, Dinophyceae, Haptophyceae, Cryptophyceae, and Raphidophyceae were analyzed by multiple regression analysis to obtain Chl a contributions of the algal classes to total Chl a. The Chl a contributions of diatoms, raphidophycean flagellates, and dinoflagellates were compared with those calculated from cell counts and observed cell sizes ; there was a good correlation for the estimated algal-classes abundances between pigment and cell count.