Study on the genetic interaction of rice organelle genomes.

1.测定了水稻线粒体DNA全长27588bp的核苷酸序列。该序列包含编码启动子蛋氨酸tRNA、NADH脱氢酶复合体亚基III和IV、核糖体蛋白S3、S12和L16的基因。除了RPS3有63bp的缺失外，所有这些基因在植物物种中都很保守。还存在一个包含核糖体蛋白S2和ATP合成酶亚单位α基因部分同源序列的ORF。除了这些基因外，还有许多短序列与水稻或其他植物的线粒体DNA片段同源。从水稻幼苗中构建了cDNA文库和基因组文库。从烟草叶绿体中分离到几种新的RNA结合蛋白，并测定了它们的N端氨基酸序列。

1. The nucleotide sequence of a 27,588 bp region of a rice (Oryza sativa) mitochondrial DNA was determined. This sequence contains genes encoding initiator methionine tRNA, NADH dehydrogenase complex subunits III and IV, ribosomal proteins S3, S12 and L16. All of these genes are well conserved among plant species, except that rps3 has a 63 bp deletion. An ORF which contains homologous sequences to portions of the gene for ribosomal protein S2 and ATP synthase subunit alpha is also present. In addition to these genes, there are many short sequences showing homology to fragments of mitochondrial DNAs from rice or other plants.2. A cDNA library and a genomic library have been constructed from Indica rice seedlings.3. Several new RNA binding proteins were isolated from tobacco chloroplasts and their N-terminal amino acid sequences were determined.